FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25708789
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3722021.4477616973712764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT85250.017.3004322
CGGCTTT88500.016.3917063
TATCGCG26000.016.0214639
CTCGGCT108600.015.4657271
ACGCAAC90900.015.02303852
TGTCGCA92700.014.90913339
AATGTCG94800.014.54246337
CGATGAC101150.013.9366524
GTCGCAG100900.013.80119140
TAACGGG13350.013.78132769
GACGATG102650.013.69858622
ATGTCGC101150.013.69817738
ACGATGA107650.013.223823
AGACGAT110600.013.09065821
TCGCAGC109300.012.93108941
CTCGACT37450.012.7873941
AAGACGA114650.012.38589120
CTAACGG12550.012.32754968
ATCGCGA10150.011.97299625
AACGCAA116950.011.8245151
Produced by FastQC (version 0.11.5)