FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004965210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21211355
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC4604662.1708466997982923Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC3360791.5844296604342345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC3180391.4993808740648582No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC2845451.3414748845606517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC2613191.2319769293380833Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC2589701.2209026721772371Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG2476581.1675727458241116Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1974630.9309306265441316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA1954920.9216384337539965Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC1918320.9043835247677483Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1843440.8690816781860471Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA1758240.8289145130049448No Hit
TCCTTCATTCCACCGGAGTCTGAAGATCGGAAGAGCACACGTCTGAACTC1547930.7297647887181182Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT1542330.7271246933541021Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAATGTTTAGGACCACTAGTAGATCGGAAGAGCACACGTCTGAACTCC1481830.6986022345107137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1473080.6944770855044385No Hit
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1433880.6759964179563258Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1336460.6300681875344597No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT1285140.605873599305655Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT1235620.5825276131581409Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCCA1225920.5779545908311845Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA1219300.5748336209544369No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1063600.5014295409227747No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATG888020.41865312234885516TruSeq Adapter, Index 2 (97% over 36bp)
GCTTCTGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC854220.402718261044615Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCC832940.3926858986613537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC791340.3730737616715198Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA791310.3730596183034983Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCCAGATCGGAAGAGCACA781660.3685101682565777No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC774710.36523362133159337No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA773940.36487060821904116Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC773410.3646207420506611No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG762670.3595574162989587Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT749160.35318818623326986No Hit
AGCTACATCTGGCTACTGGGTCTCTAGATCGGAAGAGCACACGTCTGAAC736730.34732811741635555Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAG736440.34719139819214756Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA731310.3447728822604685Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC697590.32887573660428576Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG697530.3288474498682427No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC695110.3277065515145072Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC680060.3206112952237139No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC677880.3195835438141505No Hit
TGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAGT663110.31262029229155797Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT660050.3111776687533635Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC624120.2942386283195958Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAGCGGGTGCTCTTATTTTAGATCGGAAGAGCACACGTCTGAACTCCAG583300.2749942188983212Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC567470.26753123503896853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC540590.25485877729169115Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC520270.24527900268511843Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC503040.2371559949847617Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC491870.23188994762475099Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCTGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC488980.23052746983867836Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC481810.22714720488153634No Hit
CAAGTCACTAGTGGTTCCGTTTAGAGATCGGAAGAGCACACGTCTGAACT474070.2234982159319855Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT460190.21695455099403127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA450180.21223538053085247No Hit
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC448870.21161778679391297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC429220.20235388073982075Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC422890.19936963008728106Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGAGAGGGGCTGTGCTCGCAAGGTTTCTTTAGATCGGAAGAGCACACGT420570.1982758762936173No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG407400.19206693773217223Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA405230.191043900778616Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAGCGGGTGCTCTTATTTAGATCGGAAGAGCACACGTCTGAACTCCAGT402450.18973328200862227Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA386230.1820864343649899No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC375900.1772164013095816No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT375570.17706082426134492No Hit
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC371170.17498646361818943Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA363980.171596769749033Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC357380.16848522878429975Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA357100.16835322401609892Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC356950.16828250717599136Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG353080.16645801270121593No Hit
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC344680.16249786965519175Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC336250.15852358324114607Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGATCGGAAGAGCACA333760.15734968369536034No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA332490.15675094778244955No Hit
TCCTTCATTCCACCGGAGTCTGTAGATCGGAAGAGCACACGTCTGAACTC328510.15487459429159522Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC327930.15460115584317927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTGAGATCGGAAGAGCACACGTCTGAACTCC326700.15402127775429716Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC326420.15388927298609636No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC325040.15323867805710667Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG320940.1513057510941663Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC319060.15041943336481803Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC313570.1478311970168808Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAG304740.14366833236254828Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC297760.14037764206954248No Hit
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA295220.13918017024372087Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG294560.13886901614724756No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACAC284770.13425356371622651No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC279550.13179261768048292No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG274980.1296381112852055No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG272870.12864336106769228No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA268470.12656900042453675No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTAGATCGGAAGAGCACA267750.12622955959202042No Hit
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC264300.1246030722695462Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC262590.12379690029231984Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC260320.12272671877869189No Hit
TAGCTTATCAGACTGATGTTGACCAGATCGGAAGAGCACACGTCTGAACT258160.12170839628114281Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGATCGGAAGAGCA257420.12135952653661211No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC253240.11938888392561436TruSeq Adapter, Index 5 (97% over 35bp)
TCGTACGACTCTTAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT252820.11919087677331316Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTACATCTGGCTACTGGGTCTCAGATCGGAAGAGCACACGTCTGAACT252310.1189504395169474Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA250380.11804054950756329No Hit
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC245680.11582475518419262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC244210.11513173015113838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGAA241930.11405683418150327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA241080.11365610542089367Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT241020.11362781868485064No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT240720.1134863850046355Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCACATTGCCAGGGATTACCACTAGATCGGAAGAGCACACGTCTGAACT237870.11214276504259157Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC236350.11142616772950148Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCGGGTAATGATGGAGATCGGAAGAGCACACGTCTGAACTCC234730.11066242585633969Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACT229850.10836177132483991Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC227900.10744245240344147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAA227150.10708886820290357TruSeq Adapter, Index 2 (97% over 36bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG225510.10631569741772745Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACACG224930.10604225896931148No Hit
TCCCTGGTGGTCTAGTGGTTAGGATCAGATCGGAAGAGCACACGTCTGAA224890.1060234011452828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT224160.10567924585675928Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG223090.10517479906399191Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACACGTCTGAACT219040.1032654443810874Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA214270.10101664886566653Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGT213540.100672493577143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)