FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21786175
Sequences flagged as poor quality0
Sequence length76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCTATGAATTGACAGACAAGATCGGAAGAGCACACGTCTGAACTCC7058973.2401144303669644Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC6617343.03740330737268Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA5749582.6390956650261Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC4788542.1979718789553466Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC4591732.1076347729695555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACACGTCTGAACTC3629871.666134601415806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC3477701.5962875539189416No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC3442351.5800616675483419Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC2646421.2147244754987967Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG2615121.2003575662088457Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT2015940.9253299397438972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1935060.8882054789333145Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1920740.8816325031814901Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1880710.8632584655176964Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1732160.7950730222262513No Hit
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCA1672400.7676427826362361Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC1662560.7631261568402898Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC1576540.7236424016606862Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC1446260.6638430105330559Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC1404440.6446473509002842Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC1256240.576622559949142Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTATGAATTGACAGCCAGAGATCGGAAGAGCACACGTCTGAACTCC1240330.5693197635656557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1183540.5432527738347829Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA1124560.5161805594603Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCCA1097370.5037001676521923Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC1093910.5021120045166259Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTC1087450.4991468213213196Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT1082000.4966452348794591Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC948650.4354366932240285Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC946660.43452326991773454Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG926100.4250860924416517Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC860680.39505787500559414Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC860170.3948237815954384Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA828960.3804981829072795Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC813600.37344784020141214Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT788460.3619084121007933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT785750.3606645039801617No Hit
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC774420.3554639582212114Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA738420.33893971750433477Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC726420.33343163726537584Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA721840.3313293866408399Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA695330.31916111937960656Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTCC656800.3014755917456828Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGAGATCGGAAGAGCACACGTCTGAACTCCA655750.3009936347247738Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGACCTATGAATTGACAGCTAGATCGGAAGAGCACACGTCTGAACTCCA645160.29613275391389265Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGACCTATGAATTGACAGACAGATCGGAAGAGCACACGTCTGAACTCCA641210.294319677501902Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCCTGTAGATCCGAATTTGCAGATCGGAAGAGCACACGTCTGAACTCC636170.2920062838015393Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT636020.29193743279855233Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAACAGCAACTCCATGTGGAGATCGGAAGAGCACACGTCTGAACTCCA628310.2883984912450212Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA624550.28667262610348077Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC618120.28372121310877196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG580970.2666691147023284Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG563690.25873747915822765Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTCC560500.25727324782803773Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCC520600.23895888103349947Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACACTGTCTGGTAACGATGAGATCGGAAGAGCACACGTCTGAACTCCA495540.22745617346780697Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA487430.22373362923964393Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG471580.21645837325735243Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT455170.20892607353057616Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA449800.2064612076236421No Hit
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA442510.20311504887847456Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCAGCAGATCGGAAGAGCACACGTCTGAACTC428050.1964778121905291Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC417880.19180971418801143No Hit
CTGACCTATGAATTGACAGCCATTAGATCGGAAGAGCACACGTCTGAACT417560.19166283204830586Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGAACTGAATTCCATAGGCTGAGATCGGAAGAGCACACGTCTGAACTC415760.19083662001246204Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC413690.18988647617124163Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTGAAGATCGGAAGAGCACACGTCTGAACTC412580.18937697874913792Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC410170.18827077263448036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC404990.18589311799799643Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG404090.18548001198007452Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC401620.18434626546422214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT396380.18194107042654345Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAACAGCAACTCCATGTGGAAGATCGGAAGAGCACACGTCTGAACTCC386410.17736477376134177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGACCTATGAATTGACAGAAGATCGGAAGAGCACACGTCTGAACTCCAG383900.1762126669780262Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG373650.17150784844058214Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC370070.1698646045026261Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC364160.16715187498493883No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG361460.1659125569311731No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC359110.16483389121771033No Hit
TAATACTGCCTGGTAATGATGAAGATCGGAAGAGCACACGTCTGAACTCC358420.16451717660397017Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTATGAATTGACAGCCAGAAGATCGGAAGAGCACACGTCTGAACTC355280.16307589560810928Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACACTGTCTGGTAACGATGTTAGATCGGAAGAGCACACGTCTGAACTC352620.16185493782180674Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGAAGATCGGAAGAGCACACGTCTGAACT352190.16165756494657735Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCC351840.16149691260627438Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG336170.15430427782756725Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACT336030.15424001689144606Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGACCTATGAATTGACAGCCACAGATCGGAAGAGCACACGTCTGAACTC329530.15125647342867668Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCA327890.15050370246268563Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG325450.14938372614743065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA323300.14839686177128386Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA311970.1431963160123335Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG305900.14041014542479346No Hit
TAATACTGCCTGGTAATGATGACCAGATCGGAAGAGCACACGTCTGAACT302100.1386659200157898Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC300380.1378764285148724Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC299360.1374082416945609Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACACGTCTGAACT292840.13441551809805988Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA290410.1333001318496707Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAACAGCAACTCCATGTGGACTAGATCGGAAGAGCACACGTCTGAACT289010.13265752248845886Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC287930.13216179526695254Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGACCTATGAATTGACAGCCTAGATCGGAAGAGCACACGTCTGAACTCC285590.1310877196203556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC285030.13083067587587083No Hit
TACCCTGTAGATCCGAATTTGAGATCGGAAGAGCACACGTCTGAACTCCA278830.12798483441907538Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC278480.12782418207877244Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAACAGCAACTCCATGTGGACAGATCGGAAGAGCACACGTCTGAACTC270350.12409245771687778Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA265460.12184791501950204Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG264700.1214990699377013Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA264220.12127874672814296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGTTCGTTCGGCTCGCGTGTAGATCGGAAGAGCACACGTCTGAACTCC261860.1201954909478144Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA258080.11846044567254234Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC255660.11734964949101896Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCTAGATCGGAAGAGCACACGTCTGAACTC254130.1166473692605517Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC253000.1161286917047164Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGACCTATGAATTGACAGCAGATCGGAAGAGCACACGTCTGAACTCCAG253000.1161286917047164Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC249270.11441659676377336Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT246400.11309924757328903Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGATTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC244700.11231893620610318Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA240100.11020750544783561Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC238310.10938588347885758No Hit
GTTTCCGTAGTGTAGTGGTCATCAGATCGGAAGAGCACACGTCTGAACTC237040.10880294498690109Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGACCTATGAATTGACAGCAAGATCGGAAGAGCACACGTCTGAACTCCA236230.10843114957077138Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC235700.10818787602688401Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC233960.10738920439223498Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA233670.10725609245312681Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT228700.10497482922082468No Hit
TGAGATGAAGCACTGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAG227830.10457549340350016Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT225030.1032902746810764Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG218750.1004077126893546No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC218690.1003801722881598Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTGC676500.069.897341
TTCAAGT1061600.069.85051
GATAGGC1035000.069.7893215
AGCTTAT1062950.069.772482
TAATCCA1044900.069.738957
TAATACT839500.069.714911
ATAGGCT986150.069.7081216
AAGTTCC165700.069.6987813
TAGTAGG529650.069.6852956
ATGTTGA1024250.069.6846516
AAGTAAT1121100.069.684214
CAGGATA1114100.069.68312
CCAGGAT1045750.069.67541511
GTAGGTT528050.069.6621258
GGATAGG1113100.069.6495714
GATGTTG1033100.069.6331115
TCCCTGG780300.069.632521
ATAGTTA490050.069.6316817
ACTTTGT582300.069.59891516
CTTATCA1062350.069.590824
Produced by FastQC (version 0.11.5)