FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28116658
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG780620.2776361258866541No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG469440.16696152152933683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG453550.041.549021
TGGGGGG409850.028.6384451
TATCGCG23750.022.6613949
CGCGCAT11100.021.90832537
CTAACGG14050.019.413168
ACTAACG14850.018.46272767
CGTATCG21100.017.9381333
CGCGTAT4250.017.1639231
CGTTCCG34150.016.78421636
GTATCGA24150.016.6791574
GTGCCGA34600.015.55744526
TCGCGCA17300.015.46152936
ATATCGC35250.015.2682898
AACGGGC27450.015.22895370
TGCGACA39200.015.20360761
TAACGGG20300.015.09759969
GATCGGT41350.014.779735
GATATCG37650.014.4794637
GTTCCGA39000.014.25244837
CCCGTAT33300.014.1866831
Produced by FastQC (version 0.11.5)