FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28116658
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3100491.1027235171406218No Hit
CTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAAATGTCGCAGCTGG292500.1040308560142532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG25500.025.4476839
TCGGCTT99600.023.72032
CGGCTTT110350.020.7815783
CTCGGCT125100.020.6884441
CTCGACT69500.020.266911
TGTCGCA115800.018.82753839
AATGTCG117600.018.687137
ACGCAAC122900.018.15340852
TCGACTT77000.017.8417242
CGATGAC127750.017.44369524
GTCGCAG126600.017.30418840
GACGATG131250.017.00415822
ACGATGA132850.016.8520423
TCGCAGC132600.016.7833541
AAGACGA135550.016.61826120
AGACGAT136350.016.546321
ATGTCGC134900.016.3420238
AACGCAA144800.015.54969551
CGACTTT86550.015.4728263
ACTAACG13050.015.09469367
Produced by FastQC (version 0.11.5)