FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28988524
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG447670.15443007722642244No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG341850.11792597650021781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG363200.033.4382861
TATCGCG15450.023.1492869
CGTATCG35700.019.9401653
GTATCGA38550.018.8262374
CTATCGC27500.017.2397163
CCCGTAT45300.017.1778281
TATGCCG15800.016.93849248
CCGTATC46700.016.0614952
TATCGAA45700.015.8806655
TCTATCG31050.015.59888362
TGGGGGG495700.014.8150961
TCGTATG17700.014.7251145
CGCCTAT34450.014.37568367
CGTATGC20200.013.59166146
TCGACGT14000.013.14596421
CGACGTA14550.013.12647222
ATCGCGG29000.012.811954510
TATCGCC40200.012.75383264
CGATAAC58950.012.6698971
CGCAATA28800.012.66033422
Produced by FastQC (version 0.11.5)