FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25512362
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG662660.2597407484261943No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG445270.17453107634643944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG474000.040.4401781
TCGTATG16250.030.37101645
CGATAGA18600.027.0829736
TGGGGGG449700.024.8970071
TATGCCG20850.024.34143348
CGTATGC24650.020.72770346
CGTATCG34350.019.4108143
GTATCGA35350.018.8616714
GATCGGA27450.017.9703161
CCCGTAT46250.016.3739871
TATCGCG24150.016.2485439
TATCGAA41300.016.1442175
GCGATAG31550.015.63558135
CCGTATC45600.015.5359552
ATCGGAA33050.015.3410132
TCGACGT12300.015.246098521
ACACGTC37650.014.20427413
GTATGCC37050.013.697347
GTCGGTT50150.013.6445270
GTCGTTA36700.013.62575420
Produced by FastQC (version 0.11.5)