FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13479129
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT1642721.2187137611042969No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT759970.5638123947029515No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT758260.5625437667374502No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG755880.5607780740135361No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA720950.5348639366831492No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTC670930.49775471397298743No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC601810.44647543620956515No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA599890.4450510118272479No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC585440.43433073457491206No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT567070.4207022575420118No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA533560.39584160074438046No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT476820.3537468926961082No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC465270.34517808977123077No Hit
CTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGTCGGATGGATGAAACCCAGACACAT450640.3343242727330527No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG416220.3087884981292189No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT396160.2939062308847998No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT391560.29049354746883127No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG335780.24911105161171762No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT326340.24210763173199099No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT321500.23851689526823283No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG300750.22312272551141843No Hit
CGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTC295690.21936877375385308No Hit
AGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC278840.20686796602362068No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG277960.2062151048483919No Hit
GCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTT269690.20007969357663985No Hit
CTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA263520.19550224647304731No Hit
CTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCG257460.1910064070163584No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA245620.18222245665873513No Hit
CTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGC244540.18142121794368166No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGC224270.1663831542824466No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA221320.16419458557003203No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT216640.16072255113813363No Hit
CTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG211110.1566199121619802No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT208350.15457230211239908No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT206940.15352624045663485No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT202650.15034354222739468No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG199090.1477024220185147No Hit
CCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTT198560.1473092215379792No Hit
GTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCT196520.14579577063176707No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG195580.14509839619459092No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT191630.14216793978305275No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA189800.14081028529365658No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC189550.14062481336887567No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC183610.1362180004360816No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG173210.1285023683651963No Hit
AGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTT165300.12263403666512873No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163760.12149152960847841No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT160380.11898394918544068No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC160020.1187168696137562No Hit
TTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA158670.11771532121993937No Hit
CGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG148710.11032611973666845No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG143520.10647572257821702No Hit
GTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTA137610.10209116627639664No Hit
CCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATG135900.10082253831089531No Hit
GGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATC135730.1006964174020443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCG116700.033.8013170
CGCGCAA2300.031.7327061
CTTATCG166150.031.49353470
TAACGCG1301.8365427E-730.96242770
AATGTCG129200.030.82332446
ATGTCGG128750.030.74561147
TGTCGGA131600.030.21428548
GTCGGAT132550.029.8444849
TCGGATG142350.027.8681550
GTCAACT143250.027.85376237
AGTCAAC145950.027.33696236
CGGATGG150450.026.9051551
AGTAAGT150900.026.52811232
GTAAGTC152450.026.28187433
CGACCGA11250.025.9503024
GAAACCC153650.025.91919560
ACCCAGA155350.025.77352563
GATGGAT154450.025.69892553
AACCCAG158250.025.35803262
CCCAGAC160850.025.07426864
Produced by FastQC (version 0.11.5)