FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16705214
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT1577650.9444057406268487No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT964500.5773646479476408No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT821430.4917207286299954No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG763030.4567615835391273No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA725020.4340082084551566No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT694620.415810297311965No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA639220.38264699871549085No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC609240.36470050608151444No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC548950.3286099776991782No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG509430.30495269321302915No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT496570.29725449790706066No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT455840.27287288866817266No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC438340.2623971174508749No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT401230.24018249631522232No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT376190.2251931642420145No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG374210.2240079055557145No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG370110.2215535820133762No Hit
CTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA359800.21538185622764247No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTC356090.2131609927295753No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA355210.21263421109121977No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT306300.1833559270776178No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG301370.18040475267183048No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTG300780.18005156952793303No Hit
CTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGTCGGATGGATGAAACCCAGACACAT280320.16780389643616658No Hit
GGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATT279620.16738486558747465No Hit
GGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT274840.16452348350640703No Hit
CTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG264400.15827393770591625No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGT260440.1559034203333163No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA258800.15492169091638094No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA248950.14902532825978762No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT236930.14182996997224936No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA231310.13846575087275145No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA230240.13782523228975097No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG230180.1377893153598631No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG221070.1323359281718869No Hit
GCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTT218620.13086932020146524No Hit
TTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA213220.1276367965115562No Hit
CTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCG209980.1256972822976108No Hit
AGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCC208910.1250567637146103No Hit
CGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG207720.12434441127183406No Hit
CCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGTGATTAT203200.12163866922028056No Hit
CCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTT199060.11916040105801698No Hit
GTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTA198200.11864559172962406No Hit
CGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTC194360.11634690821679987No Hit
AGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC193990.11612542048249128No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGC193900.11607154508765946No Hit
CCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAG174730.1045960859884824No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT174240.10430276439439806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG162700.031.23407270
CCCTAAC8250.029.9428041
CCGACCG12700.026.284243
CCCCGAC16100.025.284131
AATGTCG103450.024.9944746
ATGTCGG103550.024.9713847
GTCGGAT104950.024.60636149
TGTCGGA106450.024.22686248
CTCTCGT51950.024.1104321
ACTTATC170500.023.6809269
CGACTTA163650.023.45403967
GACTTAT175900.022.79705668
CGACCGA14500.022.7819734
CGCTGTG26950.022.721761
TTATGCT287100.022.6861870
GACCGAA15500.022.2096585
TCGGATG117900.021.93459150
TGGGGGG91550.021.9283261
CCTAACC9850.021.8867912
AGTCAAC119700.021.8496936
Produced by FastQC (version 0.11.5)