FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28944374
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1217000.4204616759028888No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG899320.3107063224100131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG632200.043.4007071
TGGGGGG623150.025.8946951
CGTATCG35000.024.1044563
TATCGCG41500.023.0054639
GTATCGA40000.021.3516774
CTAACGG17600.020.59032468
ACTAACG18400.019.677967
TAACGGG20300.019.37115369
CCCGTAT50500.019.0507741
TATCGAA47400.018.4577315
CCGTATC50350.018.0662062
ATCGCGG54350.017.56632810
ATATCGC56150.017.5596128
AACGGGC35700.016.02332570
TCGACGT24650.015.35213221
CGACGTA24100.015.27052422
GATATCG64550.015.11327
TCGCGGC66100.014.60128911
CGCAATA34450.014.10924922
GTCGTTA50400.013.70819120
Produced by FastQC (version 0.11.5)