FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10958438
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA661620.6037539291639922No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG555810.5071981974073312No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG486590.4440322608021326No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC486240.44371287221773764No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA413720.3775355575311007No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT387030.35317989662395316No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA369960.33760285909360443No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC368180.33597854000725286No Hit
CCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTT356370.3252014566309541No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA354780.32375051991898846No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT338380.3087848833930529No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC334540.3052807343528338No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG285500.26052983098503635No Hit
CTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG274350.2503550232250253No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA250020.22815295391551244No Hit
GACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGC211430.19293808113893604No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC210190.1918065330113653No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT209310.19100349885631512No Hit
CGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA206840.18874952798929923No Hit
CTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA205920.1879099922817467No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC200650.1831009127395711No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT184310.168190028542389No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT184160.16805314772050542No Hit
GTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAA176090.16068895950317005No Hit
CCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG167370.15273162105767263No Hit
GTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT164470.1500852585012572No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG149860.13675306644979876No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT148400.1354207597834655No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA137640.1256018421603517No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA133920.12220719777763947No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT133660.12196993768637465No Hit
CTTGTAGGCAGCAACTCACCCTCACTCAGAGGTCTTCTGGTTCTGGAAAC126800.11570992143223331No Hit
TGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGA122380.11167649988073117No Hit
CCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGAC118840.10844611248427924No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC115260.10517922353532501No Hit
CCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGA114640.10461344947153965No Hit
GGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAA110770.10108192426694389No Hit
CCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCA110630.1009541688331859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG3500.030.7150821
TGTAGGC38000.030.4719243
GTTAGGG36450.030.0640581
GTAGGCA38850.029.8064524
TAGGCAG39200.029.7219645
TTGTAGG40500.028.848142
CATTCGG99700.027.1935834
CTTGTAG44500.026.8854371
GCATTCG101550.026.5605263
CGCGTAA1358.874849E-925.7058522
TAGCGCG3250.025.61960619
TGCCGAC50750.025.46634570
AACGATT95400.025.3715770
TTCGGGC113950.023.739316
TCGGGCC114950.023.5331137
ATTCGGG115150.023.5185975
GGAGGCT184000.023.32092970
GCGCGTA2550.021.77137821
GGGCCGA123400.021.64069
ACGTCTG13050.021.2536894
Produced by FastQC (version 0.11.5)