FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31211156
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG596150.19100542126667786No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG365310.11704468748289873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG410000.034.661151
CGTATCG41800.022.0043853
GTATCGA43950.020.6119214
CCCGTAT51250.019.9176711
TGGGGGG528650.018.8428841
TATCGCG10350.017.7728799
TATCGAA54050.017.7234755
CCGTATC55000.017.2285462
GTCGGTT63300.014.24390970
GTCGTTA59300.014.22295420
CGATAAC63100.014.1412841
TACTCGA57600.013.61836317
CTATCGC23150.013.57069863
TCGATTG59650.013.32487920
TCGTTAC64750.013.18674921
ATAACGT67800.013.1053893
CGTTACC64700.013.08975322
CAATCGG66350.012.9209034
CGTCAAG61750.012.87645131
GCCGTTA24650.012.8175429
Produced by FastQC (version 0.11.5)