FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29132372
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG714830.24537308530867313No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG502350.1724370401421484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG480900.039.354871
TCGTATG30800.039.0455845
GATCGGA39250.031.8682861
ATCGGAA42600.030.2479612
TATGCCG40800.029.90631148
TCACCGC46400.027.62977830
ACCGCTC48750.026.79781332
CGTATGC46450.026.56501446
CTAACGG19150.026.5401868
TATCTCG47450.025.63360641
ACGTCTG53000.024.50894215
TAACGGG23250.024.19884169
ACTAACG22500.022.30369267
CACCGCT60450.021.72564531
TCGGAAG61650.021.6896613
ACACGTC59250.021.63036513
TCTCGTA55600.021.12811943
GTCACCG63550.020.66515229
TATCGCG62750.020.6447569
GTATGCC61050.020.49794847
Produced by FastQC (version 0.11.5)