FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29132372
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3696001.268691749508073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG23500.021.38687768
TAACGGG27250.020.05266269
ACTAACG24800.019.82419667
TCGAAGT32500.019.54711370
TATCGCG64950.019.4597649
CGACCTA15050.017.5323244
ATATCGC77050.016.4937488
CGCTCGA30250.016.41663718
CTCGACG31100.015.85705220
TAGGTCG14250.015.8344383
CGACGTA30850.015.7604322
GATATCG85250.014.9478797
AACGGGC54950.014.10843470
TACCGGT17250.014.09731144
TACGTTC29700.014.0250232
ATCGCGG93850.013.91141110
TCGCGGC100650.013.48967511
ACCGGTA18100.012.66792245
CGCTAAT29500.012.3629134
GTATGGC88450.012.2051022
Produced by FastQC (version 0.11.5)