FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13368489
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1195350.8941549041181842No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG982310.7347950841714422No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACAC927020.693436632965775No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC745860.5579239359062942No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA735020.5498153157024702No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCA719760.5384004130908138No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT606150.4534169867664177No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATA552950.41362191344137694No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT419530.31382005849726174No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC350950.26252031923727503No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT328510.24573457778212632No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT321150.24022909395369965No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT308880.23105079414734156No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC301500.2255303497650333No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG286900.2146091454314695No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG276050.2064930449507046No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT274800.20555801033310495No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG261840.19586357141783187No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC252320.18874234777019303No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA245580.18370064111209575No Hit
CTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA244260.18271324455591054No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG238130.17812783479120192No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA234510.17541997453863334No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCA230930.172742035393828No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG223270.1670121432571774No Hit
GTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAA221820.1659275031007618No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACAC214890.1607436711807894No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC210110.15716809880308838No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA208950.1563003866779559No Hit
CCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTT204490.15296418316236038No Hit
TGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC204230.15276969596189965No Hit
GACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGC199870.14950829521571213No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC195480.14622445363870218No Hit
TGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTG191270.14307525704662657No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT183990.1376296154337263No Hit
CGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA182550.1365524555542515No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA168790.12625959448371465No Hit
CTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG161700.12095607813268948No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA160670.12018560960778737No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA158380.11847262618834484No Hit
CCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCA153510.11482973131817664No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG144620.108179765117808No Hit
CCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGAC144000.10771598794747858No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG140170.10485104187915328No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT140170.10485104187915328No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG138200.10337742732181623No Hit
CGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCC136750.10229278716540066No Hit
CTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGT134460.10057980374595812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCA32150.025.2273654
TTGTAGG34550.023.9733282
TAGGCAG34250.023.7846265
GCGTTCG9350.023.73090715
TGTAGGC34800.023.3037013
AACGATT97050.022.15612870
GAGTCGT13950.021.8674185
GCATTCG88300.021.5868973
CATTCGG88500.021.5405334
CGTTCGC9950.021.25506816
TTCGCAC10350.020.76913818
GGTAGCG38550.020.37765970
CGCGTTC10900.020.0385614
AGTCGTC16150.019.1044356
GCATCAC392150.018.9291718
TTCGGGC104500.018.8761186
CACTGCG92350.018.6901681
ACCTCTA397850.018.59606611
ATTCGGG107700.018.4750355
GGAGGCT175500.018.4272370
Produced by FastQC (version 0.11.5)