FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24400117
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC6738622.761716265540858Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC6037512.4743774794194633No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG5433472.2268212894225057No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC5421392.2218704934898468Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC5291872.1687887808078954No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA4776111.9574127451929841Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGACCTATGAATTGACAGACAAGATCGGAAGAGCACACGTCTGAACTCC3073001.259420190485152Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACACGTCTGAACTC2571261.053790028957648Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG2203000.9028645231496226No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2004510.8215165525640716Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1993550.817024770823845Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1991290.8160985457569733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCA1784280.7312587886361365Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1778830.7290251927890345Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG1626660.6666607377333478No Hit
TGACCTATGAATTGACAGCCAGAGATCGGAAGAGCACACGTCTGAACTCC1622040.664767304189566Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC1569670.6433042923523686Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC1513620.6203330910257521Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1423230.5832881866918916Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC1405700.5761037949121309Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC1371290.5620014035178602Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC1365950.5598128894218007Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCCA1363940.5589891228800256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC1325350.5431736249461426Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1198830.4913214145653482Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC1157450.4743624794913893Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1061760.4351454544254849No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT1027480.4210963414642643Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC908320.3722605100623083Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA889720.36463759579513494Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG872630.35763353101954387No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG865370.3546581354507439Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC834050.34182213142666484Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA816520.33463773964690413No Hit
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC794400.3255722093463732Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC779100.31930174761047253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC753720.3089001581426843Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA732410.30016659346346575Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC663780.2720396791539975No Hit
TGTAACAGCAACTCCATGTGGAGATCGGAAGAGCACACGTCTGAACTCCA644050.2639536523533883Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA611170.25047830713270763No Hit
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC600070.2459291486184267Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG596800.24458899111016558No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC586390.24032261812515077No Hit
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA579960.23768738485967097Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA577050.23649476762754867Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG572570.23465871085782086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACACTGTCTGGTAACGATGAGATCGGAAGAGCACACGTCTGAACTCCA563080.23076938524516094Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG558770.2290030002724987Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC550190.22548662369118966Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC541090.2217571333776801No Hit
CTGACCTATGAATTGACAGCTAGATCGGAAGAGCACACGTCTGAACTCCA540630.2215686096915027Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG531160.21768748076085045No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC519540.21292520851436902Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACCTATGAATTGACAGCCAGAAGATCGGAAGAGCACACGTCTGAACTC512390.20999489469661148Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA507640.2080481827197796Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC506820.20771211875746332No Hit
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC506520.20758916852734763Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC488850.20034739997353293Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC488620.2002531381304442No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC478260.19600725685044873Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG477420.19566299620612476Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA475280.19478595123129944Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC471600.19327776174188016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG469590.19245399520010498Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC464900.19053187326929621No Hit
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCC462690.1896261399074439Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT450260.18453190203965006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTCC449670.18429009992042253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA444230.18206060241432448Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT438520.17972044970112233Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA428260.1755155518311654No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG421700.17282704013263545TruSeq Adapter, Index 13 (97% over 49bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT418440.17149098096537815Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC417110.1709459016118652Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC411220.16853197876059364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT407210.16688854401804712Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAACAGCAACTCCATGTGGAAGATCGGAAGAGCACACGTCTGAACTCC399280.16363855960198878Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC382000.15655662634732448Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTC373070.1528968078308805Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA372780.15277795594176863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC368730.1511181278352067No Hit
TAATACTGCCTGGTAATGATGAAGATCGGAAGAGCACACGTCTGAACTCC367960.15080255557790972Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACACTGTCTGGTAACGATGTTAGATCGGAAGAGCACACGTCTGAACTC362030.14837223936262273Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA361860.14830256756555715No Hit
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT360220.14763043964092468Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCC359580.14736814581667784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC345410.14156079661421295No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA333630.13673295091166981No Hit
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC331000.1356550872276555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC328900.13479443561684562Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA325030.13320837764835308Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGAGATCGGAAGAGCACACGTCTGAACTCCA321690.13183953175306495Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA314210.12877397268218016Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA310120.1270977512116028No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC307310.12594611738951908No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGATCG301390.12351989951523593No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAA299110.12258547776635662TruSeq Adapter, Index 13 (97% over 39bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC298470.1223231839421098Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC294300.12061417574350156No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG293130.12013466984605033Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA288920.11840926828342667Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGACCTATGAATTGACAGACAGATCGGAAGAGCACACGTCTGAACTCCA286500.11741746976049337Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC285690.11708550413918097No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG284170.11646255630659477No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA282230.11566747815184657Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGACCTATGAATTGACAGCAGATCGGAAGAGCACACGTCTGAACTCCAG280230.11484780995107524Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA278270.11404453511431932Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCACATGGTCTAGCGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA275510.11291339299725489Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC267140.10948308157702685Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCGGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA260750.10686424167556247Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT255240.10460605578243742No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCT253530.10390523947077794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG253490.10388884610676252Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATAAGATCGGAAGAGCACACGTCTG251310.10299540776792176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC245460.10059787828066562No Hit
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACT244130.10005279892715269Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTGG1116400.069.805041
GATAGGC994050.069.79867615
TCAGTGC767600.069.7694241
ATAGGCT888450.069.7437316
ATCCAGG1000700.069.719049
TAATCCA1000050.069.7188647
AAGTAAT1090700.069.697864
AGCTTAT650100.069.679962
GTTTCCG357200.069.671291
TCCAGGA1001000.069.6701710
CCAGGAT1001300.069.6631411
GCTTATC650850.069.636893
AGTAATC1008750.069.621195
GGATAGG1084950.069.6017214
CCGTTAC17350.069.599244
ATTGGTG661200.069.594983
CAGGATA1085250.069.58636512
TTCAAGT1039200.069.569011
CAAGTAA1091200.069.553513
ATAGGCC92700.069.5427916
Produced by FastQC (version 0.11.5)