FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26021190
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1893120.7275301398590918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT86450.019.3456362
TCGCTCG29350.019.28210624
TATCGCG18000.018.8977789
CTCGGCT105500.017.0373021
CGGCTTT95400.016.8040753
TGTCGCA92050.016.67283239
AATGTCG93650.016.53695537
AGACGAT100450.015.69139921
ACGCAAC97700.015.61349852
GTCGCAG102800.014.8623140
GACGATG107200.014.67081322
ACGATGA108500.014.49545123
ATGTCGC108800.014.39370938
TCGCAGC111300.013.88353341
AAGACGA117900.013.19215620
AACGCAA118250.013.07519551
CGATGAC119850.012.92027624
TAACGGG10950.012.53989669
TGGAACG124100.012.42851748
TTGACAG131500.012.2210888
Produced by FastQC (version 0.11.5)