FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28510738
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2283030.800761453456589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT90400.017.7743342
CTCGACT64100.016.8925651
CGGCTTT95500.016.3535483
CTCGGCT113850.015.9124311
TATCGCG18450.015.2404949
AATGTCG96200.015.16228137
TGTCGCA95950.015.09280539
ACGCAAC97950.014.75897552
TCGACTT72450.014.5618312
GTCGCAG105350.013.77957640
AGACGAT111800.013.696177521
ATGTCGC106500.013.63061538
GACGATG112350.013.16539222
ACGATGA114750.013.01118523
CGATGAC115650.012.970386524
CGACTTT77350.012.9221583
TCGCAGC113900.012.74515641
AAGACGA121000.012.65468320
AACGCAA119800.012.29812551
TGGAACG130450.011.39838548
Produced by FastQC (version 0.11.5)