FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26730357
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG575930.21545915005923788No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG541370.20253002980843093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG414200.038.987541
CGTATCG33400.021.411213
TATCGCG19400.021.114649
GTATCGA34750.021.078794
TGGGGGG471350.017.9332941
CCCGTAT45750.017.9144671
CCGTATC45550.017.0719592
TATCGAA45800.016.4479395
CTAACGG12100.016.29849668
GTCGGTT52400.015.87491570
GTCGTTA43750.015.3937120
CGTTACC44150.015.25447322
ATTCGTA26250.014.95030336
TCGTTAC46100.014.68435921
TAACGGG13700.014.47222469
CGATAAC48450.014.4073891
ATCGCGG29350.014.07482610
TCAATCG49850.013.927893
ACTAACG13650.013.648005567
ATAACGT52500.013.6216093
Produced by FastQC (version 0.11.5)