FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26730357
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3363701.2583819961701221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG19450.021.23569
CGCGCTA24400.013.8072246
GCGATAG45100.013.56996366
CGACGTA12850.013.23722122
ATAGGCG50150.012.65001162
TAGGTCG10150.012.6488453
TACCGTA31300.012.5305829
ACTATCG27150.012.4314270
CGATAGA49950.012.39101567
GCATTCG30250.012.3883533
CATTCGG31500.012.228274
GGCGATA52750.011.71613365
ACTAACG15200.011.56794367
CTAATCG36850.011.30495325
ATCGCGG38500.011.17893810
TAGGCGA61150.011.06287963
GACGCTA50350.011.0292177
CTAACGG15750.010.965713568
TCGCGGC43050.010.8846411
CGCTCGA15500.010.7496618
Produced by FastQC (version 0.11.5)