FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26475630
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312380.11798774948886957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG265050.029.390071
TATCGCG15500.018.8218529
CGCGAAA12050.018.16624536
CGTATCG38650.015.8162153
CTATCGC20700.015.15171463
GCCGTTA30700.014.37267629
GTATCGA42650.014.0884224
TGGGGGG442500.013.5839371
CCGTTAA32300.013.44571530
CCCGTAT48350.013.07879451
CCGTATC50150.013.0205082
TACCGCG34200.012.89779322
ACGGTTA32900.012.87911712
TCTATCG24250.012.6420762
TATCGAA54300.012.2809915
CCGCGAA18850.011.98122935
TACTCGA44950.011.59013817
CGATAAC54800.011.5394111
TCGATTG46100.011.45171520
TTGCACG44850.011.2284428
Produced by FastQC (version 0.11.5)