FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26475630
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3598141.3590384818038326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCG32350.018.1350823
CATTCGG35650.017.4321064
TATCGCG17750.017.2152029
CGCGCTA40800.013.62538546
ATTCGGG51200.012.5453495
TCGCGCT48350.011.85639345
GCGATAG51450.011.40283466
CGATAGA54150.011.21478267
CATATAG64100.010.83507212
TGTCTCG57000.010.7844218
TTCGGGC61500.010.4449896
CTCGCGC55850.010.3882644
ACTATCG27050.010.35180470
CGACGTA11750.010.346518535
ATAGGCG59150.010.30478962
GCTCGCG55400.010.22100743
GGCGATA60150.010.20463365
TTCGGTC59750.010.09392470
GTCTCGC60650.09.96376419
CGACCTA13100.09.8058854
Produced by FastQC (version 0.11.5)