FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27611906
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1404840.5087805238798075No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1251580.45327548196057166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG980900.048.7995221
TGGGGGG652450.033.821371
TCGTATG20550.029.2546845
CGTATGC26750.023.25579646
TATGCCG26500.022.55982448
GATCGGA27950.021.623551
ACGAGAT31500.020.50900732
GTCACGA33700.019.1686829
ATCGGAA33550.018.8356382
CGAGATT34700.018.61815833
CGTATCG27050.018.4837283
GTATCGA28200.017.2373984
TATCGCG26450.017.19655822
ACACGTC41000.016.17495513
CACGAGA39800.015.88261131
CTATCGC21900.015.76714663
GTATGCC40400.015.485085547
TATCGAA32450.015.3006895
CTCGTAT37450.015.21673944
GTCGTTA30250.015.15091820
Produced by FastQC (version 0.11.5)