FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29934373
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG267800.028.8393211
TATCGCG11750.020.098119
CGTATCG25900.018.2362563
GTATCGA30900.015.959764
TATCGAA35600.014.7306775
CCCGTAT40400.014.6192031
CGTTCCG27250.014.15284636
TGGGGGG312300.014.0614851
CCGTATC39300.014.0509412
TGCGACA48900.013.46557861
CTTTATT337450.013.4356051
GTATTGG151100.012.95912770
CTATCGC16050.012.81673263
TTCGCAC106100.012.6671749
CGACATT53450.012.52431363
GTCGTTA30600.012.25753620
CTCATAT171200.012.1759721
GCGACAT55400.011.95265562
AGATCGG34650.011.8270184
ATCGCGG20400.011.74637310
Produced by FastQC (version 0.11.5)