FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26186061
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1436300.5484979203248629No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1257060.48004929034573013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG922950.046.4941
TCGTATG20050.032.38368645
TATGCCG22200.028.9448648
TTACTCG24700.027.39210735
TGGGGGG863350.024.1465911
CGTATGC28300.023.68189446
ACGTCTG33650.021.64294215
CGTATCG31300.018.8356253
GATCGGA34950.018.7609631
ATCGGAA38000.018.343882
CGCAATA50150.017.98029922
GTATGCC37150.017.85524447
GTATCGA33050.017.8382424
GTCGGTT39700.017.38989870
CTAACGG13250.017.13531168
GACCGCA54450.016.75090619
TCGGAAG44900.016.3743823
CGTCAAG48850.016.0505331
GGACCGC57650.015.88117118
AGGACCG58900.015.77971217
Produced by FastQC (version 0.11.5)