FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26946091
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1600190.5938486587906201No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1122330.41650939277240623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG891800.048.144991
TCGTATG26400.040.00138545
TGGGGGG664950.036.5286641
GATCGGA32300.033.9573171
CGTATGC31700.033.8650246
TATGCCG32050.032.96153648
ATCGGAA40100.028.2938842
TATCTCG39600.026.4819641
CTCGTAT40850.024.82783544
CGTCTGA47100.024.75198416
ACACGTC47300.024.1334813
ACCGCTC47200.024.120532
TCACCGC49950.023.27758830
TATCGCG22900.022.8778579
GTATGCC46850.022.76853247
GTCACCG52100.021.91680129
TCGGAAG52900.021.7098433
TCTCGTA50250.020.319443
CGTATCG23500.020.0796173
CACGTCT57850.019.79253614
Produced by FastQC (version 0.11.5)