FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26946091
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5745492.1322165059117477No Hit
CTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAAATGTCGCAGCTGG273460.1014841076577675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT96150.023.7986972
CGGCTTT108550.020.7300723
CTCGGCT118750.020.7008841
TATCGCG21550.020.2780519
TGTCGCA121000.017.75070439
AATGTCG122950.017.55328637
CGATGAC128450.016.93315924
ACGCAAC127650.016.7971452
GACGATG131650.016.70561422
ACGATGA133450.016.63640823
GTCGCAG129100.016.5569440
ATGTCGC133000.016.33166938
AGACGAT135700.016.2582821
TCGCAGC137150.015.7883341
AAGACGA140300.015.62617520
AACGCAA144650.015.03628151
CTCGACT45200.014.2675951
CGCAACA154750.014.2185653
TTGACAG156150.014.2144898
CAAGACG160600.013.9960619
Produced by FastQC (version 0.11.5)