FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16922721
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT631500.3731669392883095No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT395220.23354400276409448No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT388740.229714831320566No Hit
CTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGTCGGATGGATGAAACCCAGACACAT365410.21592863228082532No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT314050.1855789030617476No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC302240.17860011992161307No Hit
CTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCG296130.17498958944013793No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC288640.17056358726235574No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA271150.16022836989394318No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA265820.1570787581973372No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT257900.15239865976635789No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA253670.14989906174072126No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGC253570.14983996958881496No Hit
GTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCT241260.14256572568914894No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG237550.14037340685342506No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT211830.12517490538312367No Hit
GCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTT197970.11698473312890993No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC194090.11469195763494536No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG174170.1029208009752096No Hit
GGGATGAATTCCAGAGGTTGTCTTTATACACATGCAGCGGAGTTCAGCAT173380.1024539729751498No Hit
CGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTC172340.10183941459532425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGTCG97300.031.93583746
ATGTCGG100750.031.08714547
GTCGGAT101450.030.806549
CTTATCG135300.030.77652770
TGTCGGA103050.030.32782648
CGCGAAA2900.028.77626836
AGTCAAC112700.027.95281236
TCGGATG114800.027.2244450
GTCAACT120050.026.21438837
AGTAAGT124200.025.27305232
GTAAGTC126800.024.92101333
CGGATGG128200.024.8148151
GACACAT138750.023.60999568
AACCCAG132800.023.52365362
GATGGAT135000.023.36366753
CGACTTA136450.022.82658867
ACCCAGA134950.022.74076563
CCCAGAC136650.022.66941364
ACTTATC146950.022.43949369
AAACCCA141050.022.4388661
Produced by FastQC (version 0.11.5)