FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13344615
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA717970.5380222659102567No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACAC505870.3790817494547426No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC446770.33479422223870825No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCA400530.3001435410463322No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG391500.29337676658337464No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATA355810.26663189608692345No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG354770.2658525555064721No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT340070.25483687614816913No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA334610.25074533810079946No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC327940.24574706726271234No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA312980.23453655275929652No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA305820.229171092609266No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT278470.20867593407528054No Hit
CCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTT259680.19459534801116407No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT247990.1858352601405136No Hit
GACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGC245450.1839318706459497No Hit
CTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG212730.15941261699944134No Hit
CGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA211200.15826608710704657No Hit
GTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAA207250.15530609163321685No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT187930.14082834161944724No Hit
CTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA176390.13218065864020806No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC175170.13126643219006318No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC144350.10817097383476405No Hit
GTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT142780.1069944693046596No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT141790.10625259702134532No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA140730.10545826912203911No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG139040.10419184067880566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGG36200.029.584421
CATTCGG107550.023.4296864
GCATTCG113650.022.2612043
GTAGGCA30250.021.4268674
AACGATT107250.021.416370
TAGGCAG31600.020.8433555
TTCGCGT4350.020.73783342
TGTAGGC31150.020.6942713
TTCGGGC125550.020.1850346
CACTGCG122900.020.1635651
TGCCGAC61050.019.93469470
GGAGGCT208550.019.89033370
ATTCGGG128300.019.7511255
ACTGCGC126900.019.3904132
TCGGGCC130250.019.3506377
ATAACAC308350.018.17777670
TGCGCAG137700.018.1737984
TTGTAGG35450.018.0861382
CAGCATT143900.017.9435621
GGGCCGA145300.017.4184259
Produced by FastQC (version 0.11.5)