FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20552045
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG821727939.98278030239813No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG8987994.37328256141907No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC8260674.019390771088716No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG5711932.779251407828272No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT5472462.662732589384657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2748701.3374338174133036No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA1723620.8386610675482659No Hit
TGGAGTGTGACAATGGTGTTTGAGATCGGAAGAGCACACGTCTGAACTCC1060360.5159389248125917Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC984710.4791299357314564No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA854420.4157347845433386Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC737470.35883047161486853No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG660050.3211602543688475No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC613420.2984715146351616Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGCGCACACG503910.24518727941671986No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTAGATCGGAAGAGC467450.22744695235924212No Hit
TGGAGTGTGACAATGGTGTTTGAAGATCGGAAGAGCACACGTCTGAACTC447740.217856665845175Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAAGATCGGAAGAGCACA447030.21751120144005132No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT429890.209171398758615No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA404110.19662763486553284Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC385990.1878109939911089Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAAGATCGGAAGAGCACAC377760.18380652630918237No Hit
TGGAGTGTGACAATGGTGTTTGTAGATCGGAAGAGCACACGTCTGAACTC366440.17829855861059082Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG365770.1779725569888544No Hit
GGCTGGTCCGATGGTAGTGGGTTATCGGAACTAGATCGGAAGAGCACACG337090.16401774130019664No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTATATCGGAAGAGCACACG336950.16394962155834128No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT333080.1620665972656249No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA319170.15529841434270894Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTAGATCGGAAGAGCACA306350.14906059226709556No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC303250.14755222655458375No Hit
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG299500.14572759061202914No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG296060.14405379124072568TruSeq Adapter, Index 6 (100% over 49bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC289070.14065266984380387Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGCTCGGAAGAGCACACGT282400.13740725071398005No Hit
GGCTGGCCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG258560.12580743181517948No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC245210.11931172785968501Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGGACTAGATCGGAAGAGCACACG233300.11351668410613154No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAAGATCGGAAGAG228670.11126386692905743No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACAGATCGGAAGA222840.10842716625036584No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG221810.10792599957814417No Hit
GGCTGGTCCGGTGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG219970.1070307115423307No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC215740.10497252219912909Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC215000.10461246070646497Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG213640.10395072607129852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT13935250.069.9506761
CTGGTCC14044150.069.6026463
GCTGGTC14041150.069.592662
TGGTCCG14162600.069.575124
GGTCCGA14235100.069.54595
GTCCGAT14061150.069.535036
TCCGATG14058800.069.515617
CCGATGG14114950.069.5067148
CGATGGT14095050.069.492079
GATGGTA14074600.069.47665410
GGGTTAT14094950.069.4670319
AGTGGGT14095500.069.4620816
GGTAGTG14218250.069.45647413
TAGTGGG14099050.069.44633515
ATGGTAG14087550.069.43571511
GTGGGTT14150250.069.41543617
TGTGACA379450.069.397656
TGGGTTA14128000.069.3956918
TGGTAGT14102800.069.3822212
GTTATCA14042950.069.3122821
Produced by FastQC (version 0.11.5)