FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17066340
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG824974548.33927485330774No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC8503294.982491852383112No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG8247114.83238351046563No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG6345243.7179852270609866No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT4963612.9084209033688535No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1969491.1540201355416568No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA1488490.8721788034224092No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC941160.5514714930090459No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC756310.4431588729628028Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC688310.40331436031392787No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG661810.38778671935517517No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGCGCACACG528270.3095391278973699No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAAGATCGGAAGAGCACA523900.30697853201096426No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT504260.2954704992400245No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAAGATCGGAAGAGCACAC472600.27691936290968067No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTAGATCGGAAGAGC470120.2754662100954276No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA402680.23594982872719047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTATATCGGAAGAGCACACG352250.20640043500832636No Hit
GGCCGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG350780.20553909039665214No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTAGATCGGAAGAGCACA335880.1968084545368251No Hit
GGCTGGTCCGATGGTAGTGGGTTATCGGAACTAGATCGGAAGAGCACACG328620.1925544668628423No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC279170.16357930288509429Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC276150.1618097377645119No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGCTCGGAAGAGCACACGT262150.15360645574856707No Hit
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG259130.15183689062798467No Hit
GGCTGGCCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG250400.14672155834232764No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC247540.1450457450162132Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAAGATCGGAAGAG243280.1425496034885043No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC238930.14000072657640714Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGGACTAGATCGGAAGAGCACACG229000.13418225583224053No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC228750.13403576865338437Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG221630.12986381379956102No Hit
GGCTGGTCCGGTGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG220740.1293423194428331No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC209740.12289688357316214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACAGATCGGAAGA203780.11940462922923134No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC200880.11770537795449991Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA199900.11713114821338379Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTATATCGGAAGCGCACACG199740.11703739641891583No Hit
GGCTGGTCCGATGGTAGTGGGTTACCAGAACTAGATCGGAAGAGCACACG195590.1146057092499036No Hit
GGCTGGTCCGATGGTGGTGGGTTATCAGAACTAGATCGGAAGAGCACACG195440.11451781694258993No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTACATCGGAAGAGCACACG194170.11377366207400064No Hit
GGCTGGTCCGATGGTAGTGGGTTGTCAGAACTAGATCGGAAGAGCACACG175390.10276954519832607No Hit
GGCTGGTCCGATGGCAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG171640.1005722375154837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT13940200.069.979851
ACTATAT125800.069.5810330
TCCCTGG96100.069.5776141
GCTGGTC14059750.069.536562
CTGGTCC14064950.069.535153
TGGTCCG14145600.069.5087664
GGTCCGA14206600.069.4800955
GTCCGAT14116850.069.44956
TTCAAGT126100.069.431761
TCCGATG14115050.069.4286657
CCGATGG14171250.069.418928
CGATGGT14152550.069.410379
GATGGTA14134300.069.40102410
ATGGTAG14140900.069.3863911
GGTAGTG14225050.069.3841913
TGGTAGT14149050.069.37862412
CGTTACC10800.069.36855
AGTGGGT14164600.069.3534516
GGGTTAT14149000.069.35223419
TAGTGGG14167650.069.3513615
Produced by FastQC (version 0.11.5)