FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27630959
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5450181.9724903504073095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG41100.018.50422
CTAACGG16600.015.13492968
TAACGGG18300.014.37258169
TCGAAGT27600.014.14342370
GATATCG60000.013.42765220
CGACGTA22250.013.42468635
ACTATCG30700.013.42166270
ACTAACG18600.013.40127767
ATATCTG120500.013.27290870
TAGGTCG10000.013.1901183
TACGTTC24300.013.14755120
ATCGCGG60050.012.78046123
GACGCTA57750.012.5047197
CGCTCGA24300.012.43384431
CGCTAAT26000.011.88908634
TCGCGGC67000.011.86976324
CCTAACT104500.011.5618037
GCGATAG51450.011.54142166
GCGCTAA41400.011.41036342
GTATGGC85950.011.3476072
Produced by FastQC (version 0.11.5)