FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19078530
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG808033142.35300623266048No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC7710224.041307165698825No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG7499733.930978959070746No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT6642553.4816885787322187No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG5683942.979233725030178No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA3267201.7125009107095777Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2345061.2291617855254047No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA1689670.8856395120588432No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC1299600.681184556671819No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC1072140.561961534772333Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1028440.5390562061123158Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC893470.46831176196488933No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTC771650.4044598823913582No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTAGATCGGAAGAGC684850.3589637147096763No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAAGATCGGAAGAGCACAC639740.3353193353995303No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG635770.33323846229243026No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC585050.3066536048636871No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAAGATCGGAAGAGCACA537820.2818980288313617No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGCGCACACG522410.27382088661967147No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC499530.26182834841049074Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG492230.25800205781053365No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC431660.22625432881883456Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT422980.22170471205066639No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACAGATCGGAAGA405380.21247968265898895No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAAGATCGGAAGAG405100.21233292082775768No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC395020.20704949490343333No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC393250.20612175047029307Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG376380.1972793501386113No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA369270.19355264792413251Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC356970.1871056103379034Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGCTCGGAAGAGCACACGT351730.18435906749629033No Hit
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG349980.1834418060510951No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT349260.18306441848507196No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTAGATCGGAAGAGCACA334940.17555859911638896No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTATATCGGAAGAGCACACG333540.1748247899602328No Hit
GGCTGGTCCGATGGTAGTGGGTTATCGGAACTAGATCGGAAGAGCACACG328840.1723612877931371No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAA274920.14409915229317982Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG266840.13986402516336427TruSeq Adapter, Index 16 (97% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACTAGATCGGAAG266500.13968581436829774No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC259780.1361635304187482No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG257480.13495798680506307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA253650.13295049461357872Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGCCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG248850.1304345775067576No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC243320.12753603133994076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC232760.1220010137049343Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG228520.11977862026057563Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG221340.11601522758828903No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGGACTAGATCGGAAGAGCACACG220320.11548059520308954No Hit
GGCTGGTCCGGTGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG216610.11353600093927571No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGT213210.11175389298861076No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTACATCGGAAGAGCACACG196670.10308446195802297No Hit
GGCTGGTCCGATGGTGGTGGGTTATCAGAACTAGATCGGAAGAGCACACG191410.10032743612846481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT14179150.069.9787451
CGTTACC497000.069.874235
TTACCAT496450.069.818047
CATTACT503050.069.7699111
TTACTGA517700.069.6271313
CTGGTCC14292000.069.600233
AACCGTT504650.069.599072
GCTGGTC14287400.069.5960542
CCGTTAC513300.069.578624
TGGTCCG14398500.069.575764
ACTGAGT445750.069.5508615
GGTCCGA14475050.069.54355
GTCCGAT14286350.069.524536
CCATTAC506200.069.5156910
ACCGTTA505250.069.5091253
TCCGATG14285350.069.5024957
CCGATGG14341100.069.501468
CGATGGT14317450.069.497159
GATGGTA14295800.069.4926610
GGTAGTG14480750.069.47954613
Produced by FastQC (version 0.11.5)