FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25359527
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9775523.854772212431249No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8176533.2242438906687814No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1018910.401785885044307No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACC976510.38506633029866844No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACCAAACAAAAAGAGC726980.2866693846458572No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT703260.27731589788721217No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG633120.24965765331506382No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA562040.22162873936883762No Hit
CTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA551370.2174212476439328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG548190.2161672810380099No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT528460.20838716747358893No Hit
TGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTTGCAACTTACCAAACAAAAAGAGCA412410.16262527293983048No Hit
CTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAACAC316390.1247617907069008No Hit
TGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCTG313510.12362612283738574No Hit
CTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTA297590.11734840322534407No Hit
CTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC272060.10728118075703857No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA258290.10185126875591963TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA75050.061.254121
TCGTATG74400.059.55953245
GTACTGG267150.057.4638821
TACTGGA279950.054.940392
ATCGGAA85500.054.8424152
AGGGGGG5476400.054.6099661
ACGTCTG85750.054.35877215
TATCTCG81100.052.68744741
CGTCTGA90000.052.09988416
CTAGTAT142350.051.5591661
TCGGAAG93850.050.036353
AACGGGC137050.049.4711470
TCGAAGT172250.049.46018270
ATCTCGT88200.048.49075342
TCTCGTA87100.048.47103543
TAGTATG154650.047.418812
ACACGTC100500.046.44952413
ACGGGCT83800.046.3109570
GCCGTCT86700.045.95622351
TGGGGGG3079250.045.8272671
Produced by FastQC (version 0.11.5)