FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30507514
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG848710.27819703696604053No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG638990.20945331697627018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG549900.040.624631
TGGGGGG477150.026.6161921
TAATGCG21050.026.57028434
TATCGCG20100.023.3223029
GATCGGA26450.021.8010331
CGTATCG28000.019.9669723
AATGCGC30100.019.50497835
GTATCGA32150.017.0654754
ATCGGAA35700.016.9249842
CGTTCCG30350.016.25422536
CCCGTAT41700.016.2440071
TGCGCAT34500.016.11253537
CGCATCG19850.016.10365739
TATCGAA35150.016.0040725
ATCGCGG32100.015.03638710
CCGTATC40700.015.016392
ATATCGC34300.014.4768548
CGCGTAT3454.461981E-914.096251
GATCGGT39200.014.084845
ATGCGCA43750.013.816812536
Produced by FastQC (version 0.11.5)