FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30507514
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3888051.2744565158603223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG20200.021.4766359
TCGGCTT106850.021.2364832
CGGCTTT115500.019.0459983
CTCGGCT133450.018.9800421
TGTCGCA125600.017.11122339
CTCGACT49700.017.034381
AATGTCG127600.016.9791337
ACGCAAC130150.016.44732752
AGACGAT135500.016.26788721
GACGATG132950.016.24039322
GTCGCAG134600.015.99343140
CGATGAC135050.015.83405324
ATGTCGC138500.015.71822638
ACGATGA141200.015.34092623
AAGACGA145350.015.30872220
TCGCAGC144250.015.21292441
ACTAACG12550.014.00703667
CGCAACA160600.013.67718253
AACGCAA158700.013.57435851
TGGAACG161050.013.48052548
Produced by FastQC (version 0.11.5)