FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22438314
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC8263263.682656370705927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC7325793.2648576002635497Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC7137673.1810188590818362No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA5164652.3017103691480565Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC5036792.244727478187532No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG4061891.8102474187677382No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG3527281.5719897671456065No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC2641151.1770715036789305Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC2272851.0129326116035278Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC2205750.9830284040057554Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1890110.8423582983997818Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1830010.8155737547838934No Hit
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC1828780.815025585255648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1716390.7649371516950872Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG1397650.622885480611422No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC1367040.6092436356849271Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1358940.6056337387916044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1336350.595566137455782Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC1300830.5797360710791373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC1070180.4769431428760646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC1019460.4543389489959005Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG981520.43743037021409004Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC954350.4253216173015495Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC916480.40844423515955786Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA885090.39445477053222444No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG873930.3894811348125354No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA810510.3612169791366678Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC810290.36111893255438Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG790430.3522680001714924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT782650.3488007164887701Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC744160.3316470212512402Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC732070.32625891588824363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC695820.3101035131249166Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG683720.3047109510990888No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC679380.30277675943032084No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC674260.30049494806071436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA660730.294465083250016Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA640520.2854581676680343Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC597890.2664594140183616No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC594370.26489066870175715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC583440.26001953622718715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC572850.25529993028888Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC567850.25307159887324865Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA544120.24249593797466243Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACACGTCTGAACTC527390.23503994105796006Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC526590.23468340803145904No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATG523180.2331636860059985TruSeq Adapter, Index 2 (97% over 37bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC515460.22972314230026375Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA510580.22754829083860756Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT507340.22610433208127848Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA490450.21857702855927588Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGAACTGAATTCCATGGGTTAGATCGGAAGAGCACACGTCTGAACTCC488080.21752079946826663Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG487620.21731579297802855Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA486370.2167587101241207Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC482820.21517659481902252Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG474720.21156669792569976Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC471970.21034111564710253No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA470390.20963696291976305No Hit
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT456320.2033664383161765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC448360.19981893470249146No Hit
TACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTC448250.19976991141134756Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC445980.19875824894865093No Hit
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC444960.19830366933986215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCA430060.19166324172128085Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC425240.18951513023661223No Hit
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCC402460.17936285230699597Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC386350.17218316848583187No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC380380.16952254077556808No Hit
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA379190.16899219789864783Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA374670.1669777862989171No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA366870.16350158929053224Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC366000.1631138596242124Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT365610.16294004977379317Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG362700.16164316088989572No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG362490.16154957097043923No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA351550.1566739818330379Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA345340.15390639421482383No Hit
GTTTCCGTAGTGTAGTGGTCATCAGATCGGAAGAGCACACGTCTGAACTC342360.15257830869110753Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG338730.1509605400833592No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC331510.14774282951918757No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA330680.14737292650419279Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGAGATCGGAAGAGCACACGTCTGAACTC330320.14721248664226733Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT330110.1471188967228108No Hit
TCCCACATGGTCTAGCGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA328780.1465261605662529Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA322620.1437808562621951Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCT319270.14228787421372213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA317190.14136088834481947Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA317060.14130295172801308No Hit
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC315880.14077706551392408Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACACTGTCTGGTAACGATGAGATCGGAAGAGCACACGTCTGAACTCCA315380.14055423237236098Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCAGAGATCGGAAGAGCACACGTCTGAACTCC313400.13967181313177093Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC310870.13854427743546152Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG306680.13667693570916245No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC295630.13175232328061726No Hit
ACCCTGTAGATCCGAATTTGTGAAGATCGGAAGAGCACACGTCTGAACTC295290.13160079674435432Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC294430.13121752374086573No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC294360.13118632710104688No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC288260.1284677627739767Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG288200.12844102279698913Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC284520.12680097087508446Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA282170.12575365510973774No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC274660.12240670132345952No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT274530.12234876470665308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC274400.12229082808984668Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT272750.12155547872268835Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA263730.11753556884888945Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA262730.11708990256576318Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA260930.11628770325613591Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT257290.11466547798555632Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA254540.11343989570695909Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG254110.1132482592052148Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC252570.11256193312920033Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACA237160.10569421570622464TruSeq Adapter, Index 2 (97% over 37bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG235880.10512376286382302Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTCC231210.10304250132162336Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGAACTGAATTCCATGGGTAGATCGGAAGAGCACACGTCTGAACTCCA230980.10293999807650432Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA230860.10288651812252916No Hit
TTTGTTCGTTCGGCTCGCGTGAGATCGGAAGAGCACACGTCTGAACTCCA230650.10279292820307266Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC225720.10059579342726017Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCA225140.10033730698304694No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGC207.929567E-570.0040054
TCCCTGG1361700.069.900951
CGTTACC66500.069.84615
TCAGTGC1119150.069.833861
GTTTCCG498300.069.833441
GATAGGC1052400.069.8087215
ATTGGTG865350.069.774073
ATAGGCT990850.069.7727416
ATCCAGG1065050.069.772469
AAGTAAT1160850.069.74474
CCAGGAT1066150.069.74283611
TAATCCA1064150.069.7393957
TTCAAGT1101150.069.725591
TCCAGGA1065200.069.71648410
AGTAATC1071600.069.700245
AGCTTAT395900.069.686352
CCCTGGT1419300.069.666762
GCTTATC395850.069.6597753
ACTTTGT1013800.069.63624616
AAGTTCC136750.069.6361913
Produced by FastQC (version 0.11.5)