FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21410131
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC7689383.5914679830777305No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA7639573.56820329590697Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC4893782.2857309934254957Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC3692351.7245807603886216No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC3650721.7051366943994877Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG3349061.564240779283415No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG3215871.5020319119018937Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG2791591.3038640445497507No Hit
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC2731381.2757418438962376Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2558341.1949202926408997Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1812980.8467860378808518Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1750750.8177203586470349Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1706910.7972440710428161Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC1664420.7773983260541469Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1455680.6799024256320525No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA1198340.5597069910501715No Hit
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1189510.5555827752758729Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC1095530.5116876678615372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC1076670.502878753988007Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT1070190.49985214943336875Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC996920.46563003281016824No Hit
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC994940.46470523697402877Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC974800.4552984752872367Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC971510.45376181957971207Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGAGATCGGAAGAGCACAC948660.4430893019757796No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC945750.4417301323378171Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA944210.44101084668748636Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG942820.44036162132777235No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA872910.4077088552143842Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG837760.3912913937798886No Hit
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACACGTCTGAACTC771120.3601659420019429Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC766550.3580314384811564No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC730340.3411188843263033Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC728180.3401100161414239Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC723420.33788676958585634No Hit
TACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTC673780.3147014840777948Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCA669880.31287991652176256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG660260.3083867165502163Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGACCTATGAATTGACAGACAAGATCGGAAGAGCACACGTCTGAACTCC641380.29956846130460385Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC633260.295775864239224Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA627760.2932069869166144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCGAGATCGGAAGAGCACACGTCTGAACTCC626140.2924503357779548Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC624880.2918618293367752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG608770.28433735412454975No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC589270.2752295163443886No Hit
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC588770.27499598204233316Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC544170.2541647222989901Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC517570.24174069742964208No Hit
TGACCTATGAATTGACAGCCAGAGATCGGAAGAGCACACGTCTGAACTCC515790.24090931531432477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA503380.23511299393730942No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT498140.23266555445176865Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG487320.2276118721552895Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC482320.22527652913473534Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA466920.21808367263142855Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG466310.21779876078292093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT461570.2155848555994356Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC441840.20636959204032895Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA436090.20368394756669167Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC435410.2033663409158963Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAG428930.20033973636125815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA428370.2000781779429561Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA427940.1998773384431884No Hit
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT416420.19449670812383166Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA413060.19292735761401927No Hit
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT408650.19086758506989052Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC407550.1903538096053686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCGAGATCGGAAGAGCACACG405870.1895691343504624No Hit
ACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA402250.1878783460035812Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC396170.18503856889058734No Hit
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA389210.18178777140597596Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC383230.17899470115339322No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC380610.17777098141062286No Hit
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT375820.17553372279693197Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGATGAAGCACTGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCA372710.1740811394381473Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG370630.17310963674159677Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC369020.17235765628897834No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC357480.16696768459753936No Hit
ACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTCC355170.16588875612204335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG351610.1642259918914088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGTTCGTTCGGCTCGCGTGAGATCGGAAGAGCACACGTCTGAACTCCA351400.16412790748454553Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC350830.16386167838020232Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG350160.1635487424154481No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG342790.16010644680315128No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC339450.1585464376654211Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA335600.15674822353959442No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA334910.15642594620275793Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGAGATCGGAAGAGCACACGTCTGAACTC334720.1563372031679769Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC331500.15483324226274No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG331370.1547725233442056Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC324960.15177861359185518No Hit
TGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCCA323330.15101729176715453Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACACTGTCTGGTAACGATGAGATCGGAAGAGCACACGTCTGAACTCCA316390.14777583565462538Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCC312130.14578612340111324Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT307050.14341341489223022Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT301550.14084453756962068No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG295860.13818691721223003No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA289420.1351789954017563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG288730.13485671806491983No Hit
TACCCTGTAGATCCGAATTTGCAGATCGGAAGAGCACACGTCTGAACTCC288510.13475396297201545Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTGAAGATCGGAAGAGCACACGTCTGAACTC285660.13342281745029957Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC282940.13215239084711813Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC282770.1320729891844193No Hit
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG281690.1315685550919796Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCGAGATCGGAAGAGCACACGTCTGAACTCC278940.1302841164306748Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG277560.12963956175700186TruSeq Adapter, Index 21 (97% over 49bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA275540.12869608317669798Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA263150.12290910317176482No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGGAGATC260660.12174610234752883No Hit
TGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTCC259920.12140047158048684Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG259340.12112957179010256Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA257880.12044765162810074Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC257260.12015806909355202Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG255490.11933135766427586No Hit
GAAGCGGGTGCTCTTATTTTAGATCGGAAGAGCACACGTCTGAACTCCAG253520.11841123251417753Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGATCGGAAGAGCA248990.11629541173755545No Hit
TGTAACAGCAACTCCATGTGGAGATCGGAAGAGCACACGTCTGAACTCCA241920.1129932367064919Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC240570.11236269409094228No Hit
TGAGATGAAGCACTGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAG238800.1115359826616661Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA238620.11145191031292617Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC237440.11090076936007538No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT234710.10962567207085282Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC229070.10699140514366773Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACACGTCTGAACT228210.10658972614413242Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGTGCACTACAGAACTTTGGAGATCGGAAGAGCACACGTCTGAACTCC228060.10651966585351579Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC224530.10487091368100455Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGAAGATCGGAAGAGCACACGTCTGAACT224230.1047307930997713Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT222380.1038667161821663Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACACG221250.10333892865952105No Hit
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC216270.10101292701104911Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC214580.10022358107010182Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTGG1513750.069.862621
GATAGGC772250.069.8273415
AAGTAAT835200.069.803664
ATAGGCT692600.069.8029916
ATCCAGG779000.069.7549449
TCAGTGC521100.069.7425461
TAATCCA777350.069.736767
GTTTCCG331450.069.735681
AGCTTAT697550.069.7286762
CAGGATA836300.069.7094712
CCAGGAT780350.069.7012211
GCTTATC698550.069.688823
AGTAATC782200.069.687115
ATTGGTG829000.069.64563
TTCAAGT804950.069.622581
TCCAGGA781100.069.6166910
CCCTGGT1571950.069.603852
GTAATCC779800.069.5917056
CCGTAGT333800.069.585385
ATAGGCG74250.069.570816
Produced by FastQC (version 0.11.5)