FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24199141
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG633780.26190185841720576No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG416710.17220032727607976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG420950.036.0101051
TGGGGGG440300.022.8386291
TAATGCG15150.021.78084834
TATCGCG14000.019.3444849
CGTATCG25750.017.529673
GTATCGA27650.016.450614
CCCGTAT32950.016.4452061
GATCGGA21850.014.7843291
AATGCGC24150.014.52664735
TATCGAA32000.014.4312185
CCGTATC37050.013.8703512
CGCATCG13150.012.68087939
GTCGTTA35050.012.68019720
CGATAAC37350.012.1829031
TCGTTAC37050.012.08959721
ATATCGC24000.011.7182698
ATCGAAG53050.011.65026956
ATCGCGC36800.011.45744367
CGTCAAG35300.011.41367631
TCGATTG35100.011.37613720
Produced by FastQC (version 0.11.5)