FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24199141
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4573821.8900753543276596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG16150.015.9061659
ACTATCG26250.011.92695670
GACGCTA38850.010.0970319
CGTAGTA23400.010.09016223
TACCGTA31000.09.8543349
CGACGTA12350.09.84141235
ACGCAAC36050.09.25519252
CGCGATT7554.9112714E-109.19800331
ACGCTAC41600.09.17931110
CGCGCTA16350.09.13569946
AATGTCG37300.08.844437
CATTCGG24800.08.8170494
CTACCGT37800.08.8160868
CTCGTAG27800.08.74293921
CCTCGTA27500.08.71187420
ATCGCGG31750.08.6376210
ATGTCGC38250.08.62480638
TGTCGCA37300.08.56506739
TCGGCTT53000.08.5120572
CCGTATG22850.08.50807811
Produced by FastQC (version 0.11.5)