FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004965310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22486286
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC14350836.382036588879106No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG7279893.2374799466661592No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC3053801.3580722045428044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG3022421.344117032043442No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2883051.2821370323227232Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC2808541.2490012801580483No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC2363591.051125116882352Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC2331121.036685204484191Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA2139190.9513309579002952No Hit
TCCTTCATTCCACCGGAGTCTGAAGATCGGAAGAGCACACGTCTGAACTC1857380.8260056818631588Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1816540.8078435007008271Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1736370.7721906587864266No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC1719630.7647461212580859Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1580440.7028461703279946Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC1526170.6787114599538581No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT1503740.6687364912106873Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG1417730.6304865107559336Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1209550.5379056372404051No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGGAGATC1164750.5179823826842725No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1152980.5127480812082529No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC1020620.4538855371669648No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC1010470.44937167480659107No Hit
TGAAATGTTTAGGACCACTAGTAGATCGGAAGAGCACACGTCTGAACTCC1002780.4459518125847906Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCCA909570.40449988050494423Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC881140.39185661874086275Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT826370.36749955061498374Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT798590.3551453539281676Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG760480.3381972460903504No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG723330.3216760651358788TruSeq Adapter, Index 22 (97% over 49bp)
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC681580.3031091928653758Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT677050.30109463163458833No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG675740.3005120543250228No Hit
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC675580.3004408998444652Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT635680.28269675125540966No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA632130.28111801121803753Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCC610960.2717033840092579Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC605260.2691685056393928No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCAC596320.26519274903823603No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGGAGATCG581380.2585486994161686No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC577510.2568276504176813Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA571540.25417269886187516No Hit
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC561100.24952986900549073Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC506760.2253640285461103Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAGCGGGTGCTCTTATTTTAGATCGGAAGAGCACACGTCTGAACTCCAG503030.22370523971811085Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC484770.2155847346244729No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA475900.21164010810856004No Hit
AGCTACATCTGGCTACTGGGTCTCTAGATCGGAAGAGCACACGTCTGAAC471390.20963444118784225Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCCGTAGATCCGATCTTGTGAGATCGGAAGAGCACACGTCTGAACTCC469410.20875390449094172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC466680.20753983116642738Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAG461300.20514726175767756Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC459850.20450242427762413Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC452380.20118039946659041Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG450210.20021536682402777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC429070.19081408108035272Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAGT421270.18734530015316891Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC420410.18696284482017173Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA405320.18025208787258154No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA397050.17657429065875974No Hit
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT396800.17646311178288848Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC393200.17486213597034211Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTTCATTCCACCGGAGTCTGTAGATCGGAAGAGCACACGTCTGAACTC393080.1748087701099239Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCGAGATCGGAAGAGCACACGTCTGAACTCC380940.16940992389761475Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA379450.16874729779742195No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA376000.16721302931039836Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA373610.16615015925706894No Hit
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC359030.15966620721625618Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC350530.1558861254366328Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG348820.15512566192567326Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA341450.15184810866498807Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGA341300.15178140133946533TruSeq Adapter, Index 22 (97% over 39bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC339510.15098536058822698Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGAGATCGGAAGAGCACAC331470.14740984794020676No Hit
CAAGTCACTAGTGGTTCCGTTTAGAGATCGGAAGAGCACACGTCTGAACT329030.1463247421117031Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC325940.14495057120593413No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACACG324290.14421679062518372No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG323310.14378096943176832Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA320660.14260247334753282Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAGCGGGTGCTCTTATTTAGATCGGAAGAGCACACGTCTGAACTCCAGT319890.14226004240984927Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAATACTGCCGGGTAATGATGGAGATCGGAAGAGCACACGTCTGAACTCC318400.14159741630965647Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG318400.14159741630965647No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA317720.1412950097672866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT308480.13718583851508426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC303230.13485108212178748Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGATCGGAAGAGCA300650.1337037161227959No Hit
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT295770.1315335044657886Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC293500.13052400027287742No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAGATC290520.12919874807249182No Hit
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC290050.12898973178585382Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT290040.12898528463081899No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA289410.12870511386362335Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC277760.12352417824802192No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA273630.12168750321862845No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC267300.11887245408156775No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA267110.11878795813590559No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC264830.11777400678795956No Hit
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG264010.11740934007510179Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC263500.11718253516832437Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA256940.11426520146546211No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC255130.11346026640415408Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA249890.11112995716589215Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG247750.11017826598843403TruSeq Adapter, Index 2 (100% over 35bp)
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACT245280.10907981869482582Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGGAGATCGG245010.10895974550888485No Hit
ACTGGACTTGGAGTCAGAAGGCTAGATCGGAAGAGCACACGTCTGAACTC241610.1074477127970355Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC241170.10725203797550203Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC238490.10606020042616199Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA228610.10166641125172918No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC227190.10103491523678033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)