FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27192968
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG825050.30340564516532365No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG484210.17806441724198696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG484850.039.1151731
TGGGGGG512700.026.0192641
CGTATCG27250.018.9788113
GTATCGA28550.017.1419984
TATGCCG17250.016.72152948
TCGTATG18950.015.766973545
TATCGCG15400.015.325889
TATCGAA33300.015.0094615
CCCGTAT40100.014.9796871
CCGTATC40850.014.10492
TTAGCGC22150.014.01174961
CTTAGCG24350.013.31168560
GTCGTTA31200.012.6823220
TCGTTAC31300.012.64189321
CGTATGC23950.012.47636346
CGATAAC36300.012.2434641
GTCGGTT55450.011.9635370
CGTTACC35550.011.13067922
CCGGTTA36250.011.01335727
CTCAGAT140100.011.003881
Produced by FastQC (version 0.11.5)