FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004965314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22882024
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC6100382.666014160285821Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC5690182.4867468017689345No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA5066882.2143495697758206Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC4041791.7663603534372658Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGACCTATGAATTGACAGACAAGATCGGAAGAGCACACGTCTGAACTCC3976401.7377833359496522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC3709911.621320736312487Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC2755571.2042509875874619No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG2635311.1516944480086202Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG2476811.08242610006877No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC2331761.0190357286575698Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC2298261.0043954153705983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC2097650.9167239751168865Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG2080710.9093207838607283No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT1814720.7930766963621749Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC1757380.7680177243062065Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACACGTCTGAACTC1735920.7586391833170003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC1733260.757476698739587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC1515380.6622578492182335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC1389790.6073719702417932Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCA1321180.5773877345815213Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC1309380.5722308481102895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC1301220.5686647300081497Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1272910.5562925727199657Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC1111610.4858005568038911Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC1106040.4833663315797589Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTATGAATTGACAGCCAGAGATCGGAAGAGCACACGTCTGAACTCC1080040.4720037003719601Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1067690.4666064505482557No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA1023250.44718509166846426Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG1001290.4375880385406466Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC963860.4212302198441886Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC921940.4029101621429993Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC880660.3848697999792326Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA876500.3830517789859848Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT811470.3546320902381712Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA809550.35379300362590305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG796290.3479980617099257No Hit
TGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCCA786320.34364092966601206Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC781870.3416961716323696Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC763540.3336855166308715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG751730.3285242599168675No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG745130.3256398996871955TruSeq Adapter, Index 19 (97% over 49bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA743400.32488384768759965No Hit
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC728230.3182541894021263Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG681570.2978626366268998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC677420.2960489858764242Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA676580.29568188548355684Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGAACTGAATTCCATAGGCTGAGATCGGAAGAGCACACGTCTGAACTC671680.293540466525164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC663860.29012293667727995Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA650480.2842755518480358No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC629880.2752728517372414No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC612980.26788714145217224No Hit
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT590770.2581808322550488Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC562930.24601407637715964Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC556970.2434094116849104No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG551450.2409970376746393No Hit
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA543370.2374658815146772Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA538270.23523705770083975Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG534230.23347147962085874Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC526690.23017631657059706No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT521110.2277377211036926Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA516900.22589784889658363Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC508050.2220301840431598Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC505710.22100754723445795No Hit
TTTGTTCGTTCGGCTCGCGTGAGATCGGAAGAGCACACGTCTGAACTCCA497560.21744579937509026Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA484960.2119392934820801No Hit
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACACGTCTGAACT460560.2012759011178382Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT456520.19951032303785715Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA452250.19764422937411483No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT449970.1966478140220463Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC447790.1956951010977001Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA425530.1859669406867155Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC423040.18487875023643013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAACAGCAACTCCATGTGGAGATCGGAAGAGCACACGTCTGAACTCCA400020.17481845137475602Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA386580.1689448450888785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC379140.16569338446633916Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTCC376930.16472756081367626Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGACCTATGAATTGACAGACAGATCGGAAGAGCACACGTCTGAACTCCA371950.16255117991310558Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC371300.16226711413291062No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC369100.16130566072301997No Hit
TGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG353400.1544443795706184Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCC352640.1541122411199289Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG351050.15341737251914428Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACT347790.1519926733753972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG347130.15170423735243002Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC344070.15036694306412754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC340980.1490165380475084No Hit
CTGACCTATGAATTGACAGCTAGATCGGAAGAGCACACGTCTGAACTCCA338940.1481250085219734Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC337440.14746947210613887Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG328170.1434182570562814Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA328080.1433789248713313Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA317090.13857602806465025No Hit
TGAGATGAAGCACTGTAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG306360.13388675757004714Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG305740.13361580251816885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC304010.132859750518573Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA302270.13209932827620494Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTC293160.12811803711070313Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG286440.12518123396776437No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC285810.12490590867311388No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA277320.12119557255949036Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT273560.11955236127713179Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC273530.11953925054881509Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACACTGTCTGGTAACGATGTTAGATCGGAAGAGCACACGTCTGAACTC272850.11924207404030343Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC268390.11729294576388871No Hit
TGACCTATGAATTGACAGCCAGAAGATCGGAAGAGCACACGTCTGAACTC268100.11716620872349404Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGAAGATCGGAAGAGCACACGTCTGAACTCC267350.11683844051557676Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC266310.11638393526726482Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC266240.11635334356785922Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC261800.11441295577698896No Hit
TGTAACAGCAACTCCATGTGGAAGATCGGAAGAGCACACGTCTGAACTCC261500.11428184849382206Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG256300.11200932225226229No Hit
CTGACCTATGAATTGACAGCCATTAGATCGGAAGAGCACACGTCTGAACT256210.11196999006731223Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGTAGTCTGCACATTGGTTAGATCGGAAGAGCACACGTCTGAACTCCA255760.11177332914256186Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT255190.11152422530454474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG254590.11126201073821092No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC254170.11107846054177724Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC253540.11080313524712675No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC250660.10954450532872442No Hit
CTGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCC244960.10705346694855317Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC244850.10700539427805861Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC241660.10561128683371716No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT238800.10436139740085928Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAACACTGTCTGGTAACGATGAGATCGGAAGAGCACACGTCTGAACTCCA237440.10376704438383598Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA235980.10312898893909035No Hit
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC230870.10089579488248067Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTCATCAGATCGGAAGAGCACACGTCTGAACTC229860.10045440036248542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA229260.1001921857961516Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)