FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25049114
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC9896193.950714584156549No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC9186363.667339291920664No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA6383042.548209888780897No Hit
ATGACCTATGAATTGACAGACAAGATCGGAAGAGCACACGTCTGAACTCC6127122.4460426025447446Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG5901542.355987521155439No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG4112921.641942305823671No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC3471151.3857376352712514Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC3290771.3137271042800156Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC3167111.264360088744057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACACGTCTGAACTC2142830.8554514143693865Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA2140870.8546689515645144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA2034150.8120646502706642No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT1860820.7428685900826671Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC1741770.6953419590010249Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1705270.6807705853388667Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG1637310.6536398852270783No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1539940.6147682508850413Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCA1413150.564151690155588Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC1312510.5239746204197083Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA1293210.5162697570860191No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1274160.5086646976815228No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1255470.5012033559350643No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC1245110.4970674811093119Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACCTATGAATTGACAGCCAGAGATCGGAAGAGCACACGTCTGAACTCC1217450.48602517438341336Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC1141920.455872411295665Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC1074760.42906108375729374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC1072420.4281269189800486No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1066510.42576755409392925Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1022410.40816214098430786Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC979170.39090005339110995No Hit
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC939590.3750990953212956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCCA937440.37424078153023693Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC907070.36211660021188774No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC902180.3601644353568753No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC870900.34767696773626405No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT852370.3402795005044889Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA822180.32822717801515855No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACAC815990.3257560327283432No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC801980.3201630205363751No Hit
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC782800.3125060630886985Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC755490.3016034818636699Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA743980.2970085089636304Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC734330.29315607729678583Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA733430.29279678315169155Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT730990.2918226968027691Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG729620.29127577127079224No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGATCGGAAGAGCACA716790.28615383362461444No Hit
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC691580.2760896054048059Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC595270.23764113972254666No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC582370.23249125697619483No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC581520.23215192361693912No Hit
TTTGTTCGTTCGGCTCGCGTGAGATCGGAAGAGCACACGTCTGAACTCCA577230.23043928819198953Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG569280.22726552324365645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC564140.22521355445945115Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG560000.22356080139201728No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC550180.21964050305332156No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC535980.21397163987516682No Hit
ATGACCTATGAATTGACAGACAGATCGGAAGAGCACACGTCTGAACTCCA530460.21176796911858842Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC527590.2106222200114543Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA526660.21025094939485683No Hit
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA524720.2094764709043202Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG495940.19798704257563762Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG490480.19580732476206542No Hit
TGTAACAGCAACTCCATGTGGAGATCGGAAGAGCACACGTCTGAACTCCA479330.19135606952006365Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT477120.19047380278599874Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC474890.1895835517375984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT464640.18549159064069093No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG453980.18123595109990717TruSeq Adapter, Index 15 (97% over 49bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA447740.17874484502725327No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA434210.1733434563793354No Hit
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG422610.1687125540647865No Hit
TGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTCC419260.1673751814136021Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG397700.15876809056000943Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG395330.15782194931126106No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG389220.15538274128178745No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC373930.14927873297235184No Hit
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC373080.1489393996130961Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACACTGTCTGGTAACGATGTTAGATCGGAAGAGCACACGTCTGAACTC370890.1480651171933666Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA366940.14648821511211932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA366350.1462526778392242No Hit
CTGACCTATGAATTGACAGCTAGATCGGAAGAGCACACGTCTGAACTCCA361240.14421268552652203Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG358900.1432785207492768Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA354280.14143414413779265No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA347870.13887517139328762Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGATCGGAAGAGCA341690.13640801826364No Hit
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA338980.13532614367118934Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGACCTATGAATTGACAGCCATTAGATCGGAAGAGCACACGTCTGAACT338300.13505467698378473Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC336250.13423628476440325Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC333280.133050614085592No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGATCG331450.13232004932390024No Hit
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG327090.13057946879877666Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT318490.12714621363454212Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAACAGCAACTCCATGTGGAAGATCGGAAGAGCACACGTCTGAACTCC318400.1271102842200327Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACACTGTCTGGTAACGATGAGATCGGAAGAGCACACGTCTGAACTCCA310180.1238287310281713Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCAC308350.12309816626647954No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC307640.1228147231075718No Hit
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA304490.12155719359974168Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA299400.11952518560137497Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC298430.11913794635610665Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC297300.1186868325961549Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGACCTATGAATTGACAGAAGATCGGAAGAGCACACGTCTGAACTCCAG293800.1172895775874548Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACT287930.11494618133000632Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG281180.11225147524179897Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTC278990.11137719282206947Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCCAGATCGGAAGAGCACA276020.11019152214325822No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT273790.10930127109485789No Hit
TTTGTTCGTTCGGCTCGCGTGTAGATCGGAAGAGCACACGTCTGAACTCC270370.1079359533434995Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC262570.10482207075268211No Hit
TGACCTATGAATTGACAGCCAGAAGATCGGAAGAGCACACGTCTGAACTC259930.10376814126040546Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC256850.10253855685274937Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT253280.10111335674387524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT252470.10078999201329038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTGG2191900.069.878261
ATTGGTG1594950.069.8497853
AGCTTAT842500.069.773362
GTTTCCG463600.069.732691
GCTTATC844950.069.707333
CCCTGGT2279550.069.7050552
TTGGTGG1614500.069.636764
GATAGGC479800.069.6246515
CCTGGTG2280400.069.61883
GGTGGTC2326650.069.576566
ATAGGCT469250.069.57152616
CCGTAGT468650.069.527315
TCAGTGC481350.069.525571
TAGTGTA702800.069.500748
TCCCTGT892950.069.489721
TGTTGAC563550.069.4876817
TTTCCGT465250.069.48692
ATCCAGG482950.069.4788069
TAATCCA482650.069.449897
CAGACTG852800.069.44349
Produced by FastQC (version 0.11.5)