FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20825369
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC8552914.106966844140913No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG6906463.3163686079223855No Hit
ATGACCTATGAATTGACAGACAAGATCGGAAGAGCACACGTCTGAACTCC5137822.4670967414791063Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC4659762.2375401847621523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA3243491.5574706023216205Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC3177731.525893730862584Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG2693761.293499289256291No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC2059430.9889044463029681No Hit
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC1951650.9371502612990915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1947520.9351671031615334Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC1706950.8196493421076957Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1639990.7874962503665601Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1587860.7624642809450339Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1581820.7595639721918013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1397690.6711477717393627Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC1289540.619215918815172Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT1256000.6031105619304993Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACACGTCTGAACTC1169380.5615170612343051Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT1097280.5268958259515113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC1041310.5000199516272676Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1011100.4855136060254202No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA1007540.4838041525218593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC1002940.4815953081071456Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC967320.4644911694001676Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG936960.4499127962630578No Hit
TACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTC934500.44873154468475446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA896410.4304413525637889No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT882040.4235411146856509Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC867070.4163527666664634No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG799780.38404121434775057No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC796440.38243740122924114Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC725120.3481907091298118Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT721680.34653887765446073Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCA701090.33665189798077527Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG697460.3349088316274252No Hit
TGACCTATGAATTGACAGCCAGAGATCGGAAGAGCACACGTCTGAACTCC658710.3163017183513051Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC643930.30920460521011656Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC487100.23389741617543489Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA473260.22725167558855738No Hit
ACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTCC472790.22702598931140186Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC447950.21509822947194837Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC435900.20931201747253553No Hit
ATGACCTATGAATTGACAGACAGATCGGAAGAGCACACGTCTGAACTCCA433860.20833244299296688Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGATCGGAAGAGCA424780.2039723761917496No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT419460.20141779960777648No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG385620.18516838765257893TruSeq Adapter, Index 18 (100% over 49bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG380800.18285390285281378Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC380700.1828058844959722No Hit
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC371770.1785178452300173Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA363530.17456113262626943Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG361180.17343270124049182No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC345560.16593223390183384Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG344840.16558650173257433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAACAGCAACTCCATGTGGAGATCGGAAGAGCACACGTCTGAACTCCA342570.16449648503227002Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG341300.1638866519003817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA337300.1619659176267177Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA336940.16179305154208792Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCCA332880.15984350625431895Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA329180.15806682705117975No Hit
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA328980.15797079033749653Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT328240.1576154544968687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA321880.15456148700174294Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC321490.1543742154100607No Hit
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC318910.1531353418035474Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA317420.15241986828660756Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAACAGCAACTCCATGTGGAAGATCGGAAGAGCACACGTCTGAACTCC312050.1498412825242136Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGAGATCGGAAGAGCACACGTCTGAACTC303140.14556284692962704Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC296760.14249927576313293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA295880.14207671422292686Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC291930.14017998912768365No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG284400.13656420685751117No Hit
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACACGTCTGAACT278670.13381275501048745Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA275030.1320648868214532Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG274250.13169034363808874No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC271080.13016816172620999Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC270140.12971678917189897No Hit
TAACACTGTCTGGTAACGATGTTAGATCGGAAGAGCACACGTCTGAACTC258640.12419467813511492Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCA257600.12369528722396227No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGATCG257470.1236328633600682No Hit
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC256100.12297501187133826Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA255140.12251403564565891Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTCC254930.12241319709629153Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT250760.12041083161599682No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA249950.12002188292557985Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC248460.11930640940864001Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCTGGTAATGATGAAGATCGGAAGAGCACACGTCTGAACTCC243700.11702073562297984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACG243480.11691509523792831No Hit
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACT243070.11671821997487775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCCAGTTTTCCCAGGAATCCCTAGATCGGAAGAGCACACGTCTGAACTC238960.11474466550868798Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGAAGATCGGAAGAGCACACGTCTGAACT238220.11438932966806015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT237450.11401958832037981Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGACCTATGAATTGACAGCCATTAGATCGGAAGAGCACACGTCTGAACT235100.11289115693460222Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAGCGGGTGCTCTTATTTTAGATCGGAAGAGCACACGTCTGAACTCCAG234840.11276630920681405Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCTGTAGATCCGAATTTGTGAAGATCGGAAGAGCACACGTCTGAACTC234490.11259824495786847Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAACAGCAACTCCATGTGGACAGATCGGAAGAGCACACGTCTGAACTC232430.11160906680693149Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC227560.10927057282874555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACAC226710.10886241679559196No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAGATCGG224120.10761874135339451No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC223450.10729701836255578No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC221070.1061541814697257Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG215990.1037148489421724Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGA215780.10361401039280504No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA214040.10277849098376121Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCACATTGCCAGGGATTACCACTAGATCGGAAGAGCACACGTCTGAACT208400.10007025565789494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT1253900.069.698342
TAATCCA498350.069.612977
GCTTATC1257150.069.598063
TCCCTGG508300.069.579921
GATAGGC492050.069.5704615
AAGTAAT554800.069.559984
TGTTGAC841950.069.5198517
ATCCAGG499050.069.4941259
CTTATCA1254200.069.485324
ATAGGCT510000.069.4825416
CAGGATA551650.069.4674712
AAGTTCC98300.069.46133413
ATTGGTG519000.069.442023
CAAGTAA554550.069.408473
TCCAGGA499450.069.3822510
CAGACTG1263150.069.379739
CCAGGAT499950.069.34785511
GTTTCCG203050.069.33791
AGTAATC503750.069.320695
GTTGACA660350.069.3035818
Produced by FastQC (version 0.11.5)