FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18879565
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG851239845.08789265006901No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG9454075.0075677061415345No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC7797054.12988858588638No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG7764214.112494117316793No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG6060073.209856794899671No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT4879332.584450436225623No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2519921.3347341424444896No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA1613480.8546171482234892No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC936290.4959277398605318No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC585010.30986413087377807No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGCGCACACG551880.29231605707017083No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAAGATCGGAAGAGCACAC492890.26107063377784395No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTAGATCGGAAGAGC464080.24581074828789753No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGAGATCGGAAGAGCACAC452750.2398095506967454No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAAGATCGGAAGAGCACA426800.2260645306181578No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT425730.2254977802719501No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC401600.2127167654551363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTATATCGGAAGAGCACACG367940.194887964844529No Hit
GGCCGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG351160.18600004820026309No Hit
GGCTGGTCCGATGGTAGTGGGTTATCGGAACTAGATCGGAAGAGCACACG341280.18076687677920544No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA332240.17597863086358187Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG275280.14580844420938724No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTAGATCGGAAGAGCACA263210.13941528843487655No Hit
GGCTGGCCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG259930.13767796027080073No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGCTCGGAAGAGCACACGT259910.13766736680638564No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGGACTAGATCGGAAGAGCACACG258900.13713239685342327No Hit
GGCTGGTCCGGTGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG234040.1239647205854584No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAAGATCGGAAGAG232780.12329733232730733No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG225830.11961610344306131No Hit
GGCTGGTCCGATGGTAGTGGGTTACCAGAACTAGATCGGAAGAGCACACG221350.11724316741407972No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTACATCGGAAGAGCACACG210920.11171867572160694No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACAGATCGGAAGA209300.11086060510398413No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA206840.10955760898092727Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC206800.10953642205209707No Hit
GGCTGGTCCGATGGTGGTGGGTTATCAGAACTAGATCGGAAGAGCACACG205760.10898556190251205No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTATATCGGAAGCGCACACG203350.10770904944049292No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC202280.10714229909428527Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTGTCAGAACTAGATCGGAAGAGCACACG189270.10025125049226505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT15198350.069.972761
CTGGTCC15314750.069.621523
GCTGGTC15309050.069.6208652
TGGTCCG15409400.069.59784
GGTCCGA15474650.069.572385
GTCCGAT15373400.069.547556
TCCGATG15368950.069.5372547
CCGATGG15432150.069.526318
CGATGGT15409750.069.515719
GATGGTA15389150.069.50590510
ATGGTAG15395600.069.4874411
GGTAGTG15497100.069.480313
AGTGGGT15419650.069.4752816
GGGTTAT15397100.069.4736519
TAGTGGG15424750.069.47227515
TGGTAGT15411400.069.4693812
GTGGGTT15446900.069.456517
TGGGTTA15422950.069.4400418
GTAGTGG15441700.069.4268114
GTTATCA15329550.069.4256821
Produced by FastQC (version 0.11.5)