FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004965325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22813127
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC5378132.3574716434095158No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC3998011.752504161310284Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC3216871.4100960381275218Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC2980581.3065197068337016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG2945651.2912083468434643No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC2823681.237743514950844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAATGTTTAGGACCACTAGTAGATCGGAAGAGCACACGTCTGAACTCC2636841.1558433002192114Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2487511.090385373298452Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA2115890.9274879327152301Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG2081500.9124132785479168Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1905210.8351375942456287No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1895310.8307979874920259No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1879970.8240737887445242Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC1861960.8161792112058992Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCCA1834560.8041685824130993Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT1608370.7050195266961868No Hit
TCCTTCATTCCACCGGAGTCTGAAGATCGGAAGAGCACACGTCTGAACTC1605110.7035905248763136Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT1536690.673599020423636Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC1454770.6376898703978635Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCC1415260.6203708943539393Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA1192040.5225237206631077Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1183200.518648758673022No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC1013790.44438888189242975No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1010270.44284591060225986No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT938200.4112544501242639Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC922820.40451271761210117Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG876650.3842743697521168No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC867730.380364340232709Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAGT865000.37916766079459424Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG864130.37878630141321706No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC835020.3660261041811585No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC817950.3585435701120675No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT801740.35143801198318847No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC782480.3429955042989065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTAGATCGGAAGAGCACACGTCTGAACTC766720.336087201022464Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC723850.31729538874701396Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAG704280.30871699438660904Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC687970.30156760184607745Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT669200.29333988277889306Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC666850.29230977410505804No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG654110.2867252700605226Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT647630.2838848001854371Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC640790.2808865264284024Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA623530.27332070697717153No Hit
AACCCGTAGATCCGATCTTGTGAGATCGGAAGAGCACACGTCTGAACTCC608370.26667541017064433Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC595580.26106898892028263Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA594130.26043338995132054TruSeq Adapter, Index 7 (100% over 35bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG592170.2595742354829305No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC575980.25247744423638196Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC562760.2466825350159143No Hit
TCCTTCATTCCACCGGAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC556560.24396480149345592Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC552930.24237361235046823Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC544550.23870028865398416No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC531820.23312016805061403No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG492940.21607734880010093Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA477680.20938821758192117Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC466980.20469793553509782No Hit
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC460780.20198020201263947Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC457750.20065201933956708Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGAGATCGGAAGAGCACACGTCTGAACTCCA455640.19972711325369819Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG448610.19664555411452364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC448140.19643953237975664No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC442210.19384015176876015No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC425240.1864014521113217Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATG423210.18551161355477486TruSeq Adapter, Index 7 (97% over 37bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC422350.1851346376145629Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCACATTGCCAGGGATTACCACTAGATCGGAAGAGCACACGTCTGAACT419420.18385028935314304Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGAAATGTTTAGGACCACTAGTAGATCGGAAGAGCACACGTCTGAACTC416330.1824958060330791Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC406960.17838852166123478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC400370.17549983393333146Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC398260.17457492784746256No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA395140.17320729420390288No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG380670.16686445483777826No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG379870.16651377954455782No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC370660.16247663023135758No Hit
TGAGGTAGTAGGTTGTGTGGTTTAGATCGGAAGAGCACACGTCTGAACTC365000.15999560253182302Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC358130.15698417845129253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC356030.1560636558065889Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTAGATCGGAAGAGCACACGTCTGAAC351800.15420946019368587Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA348380.15271032331516848Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC341970.14990053752823976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT341180.14955424567618458Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAAATGTTTAGGACCACTAGAAGATCGGAAGAGCACACGTCTGAACTCC339820.14895809767770984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA326770.1432377069570515No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG325770.142799362840526No Hit
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT323300.1417166528727079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT320920.14067339387537708Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC318340.13954246605474122Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA313830.13756553408921102No Hit
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC311030.13633817056293948Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAGCGGGTGCTCTTATTTTAGATCGGAAGAGCACACGTCTGAACTCCAG310550.13612776538700722Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCAAGATCGGAAGAGCACACGTCTGAACTCC306960.13455411000868053Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA305930.1341026155686592Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG301510.13216513457361634Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT298120.13067914801859473No Hit
TGAAATGTTTAGGACCACTAGCAGATCGGAAGAGCACACGTCTGAACTCC297080.13022327013740817Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC296410.12992957957933604Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG295220.12940795008067066No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT290950.12753622070310658Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATACTGCCGGGTAATGATGGAGATCGGAAGAGCACACGTCTGAACTCC288460.126444743852958Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACT272690.11953205713535017Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA257110.11270265579988224No Hit
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC253700.11120790236253013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGCAGATCGGAAGAGCACACGTCTGAACTCCA248810.10906439963272023Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT247810.1086260555161947No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC247630.10854715357522009Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA244320.10709623454952055Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCTGGTAATGATGAAGATCGGAAGAGCACACGTCTGAACTCC243810.10687267905009253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTAGATCGGAAGAGCACACGTCTGAACTC243650.10680254399144844Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG240270.1053209408775921Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC238780.10466780814396903Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA238230.10442671887987999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC237220.10398399132218919No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA236640.10372975173460437No Hit
CAAGTCACTAGTGGTTCCGTTTAGAGATCGGAAGAGCACACGTCTGAACT235540.10324757320642629Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG233840.10250238820833286Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCAC233780.10247608756134134No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG230630.10109530359428587No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACACGTCTGAACT230490.1010339354179723Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)