FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27812946
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG237500.022.97581
TATCGCG16100.019.2056929
TGCGACA47100.015.45336661
GTGCCGA39100.015.28628326
CGTTCCG32450.014.67406936
CGACATT50850.014.59644563
CGTATCG22800.014.3241823
GTATCGA24300.014.1548654
CGGTACG8900.014.06891868
TAATGCG15350.014.038609534
GCCGAAA42550.013.72156628
CGCGTAT4559.458745E-1112.9859011
TATCGAA28600.012.8769875
CTAACGG11400.012.86653768
GATCGGT39200.012.5856825
CTATCGC21050.012.41559663
GTTGCGT58300.012.40577448
GACCACG78000.012.37035670
TAACGGG13800.012.32240869
CTGCGAC60300.012.23958960
Produced by FastQC (version 0.11.5)