FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28161734
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG225150.023.0756651
TATCGCG15600.021.3770649
CGCGTAT3600.018.3415531
CGTATCG23300.016.1020683
ATCGCGG22650.015.03006410
CTATCGC16600.013.64206663
GTATCGA26750.013.3760294
CCCGTAT36200.013.0561561
CGTTCCG29400.012.41186336
ATATCGC27050.012.3283628
TATCGAA32000.011.941355
TGGGGGG295350.011.8018191
TCAATCG30050.011.6759083
AGATCGG34950.011.6292714
GATCGGT36500.011.6112155
TTCGCAC81100.011.3936329
GATATCG30900.011.3544047
CTTTATT293300.011.2444741
GTCGTTA32950.011.17556820
TGCGACA42450.011.15497461
Produced by FastQC (version 0.11.5)