FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28161734
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2564650.9106861104504432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT105750.018.8723582
TATCGCG21400.018.3335659
CGGCTTT110000.017.8286483
CTCGGCT131500.016.734391
TGTCGCA114600.016.18347239
AGACGAT120650.015.859570521
AATGTCG119450.015.61393637
ACGCAAC123950.015.27922652
GTCGCAG122750.015.222548540
CTCGACT52750.015.0681321
ATGTCGC127050.014.62520138
CGATGAC132250.014.4691824
GACGATG138400.013.97600922
AAGACGA139250.013.96558820
TCGCAGC135400.013.90293441
TACCGGT14500.013.65467544
ACGATGA141800.013.59228323
AACGCAA142250.013.33705951
TAGGTCG10250.013.2069753
TCGACTT61200.013.1008432
Produced by FastQC (version 0.11.5)